In vitro characterization of engineered red blood cells as viral traps against HIV-1 and SARS-CoV-2
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SciScore for 10.1101/2020.12.20.423607: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources 2-3 ⨯ 105 cells were collected for each condition and samples were stained with the following antibodies: APC-conjugated anti-human CD4 (Invitrogen) anti-human CD4suggested: NoneBrilliant Violet 421-conjugated anti-human CD71 antibody (BioLegend) and the nuclear stain DRAQ5 (Abcam) were diluted 1:100 and 1:1,000 in PBS+ (PBS supplemented with 2% FBS). anti-human CD71suggested: NoneExperimental Models: Cell Lines Sentences Resources TZM-bl reporter cells (NIH … SciScore for 10.1101/2020.12.20.423607: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources 2-3 ⨯ 105 cells were collected for each condition and samples were stained with the following antibodies: APC-conjugated anti-human CD4 (Invitrogen) anti-human CD4suggested: NoneBrilliant Violet 421-conjugated anti-human CD71 antibody (BioLegend) and the nuclear stain DRAQ5 (Abcam) were diluted 1:100 and 1:1,000 in PBS+ (PBS supplemented with 2% FBS). anti-human CD71suggested: NoneExperimental Models: Cell Lines Sentences Resources TZM-bl reporter cells (NIH AIDS Reagents Program) were added, and luminescence was measured after 48 hours. TZM-blsuggested: NoneSARS-CoV-2 neutralization assays: Lentivirus-based SARS-CoV-2 pseudovirus was generated by transfecting HEK293T cells with a luciferase-expressing lentiviral backbone plasmid, accessory plasmids (pHDM-Hgpm2, pHDM-tat1b, pRC/CMV-rev1b), and a plasmid encoding the SARS-CoV-2 Spike protein with a 21-residue cytoplasmic tail deletion (Wuhan Hu-1 strain; HEK293Tsuggested: None1.25 ⨯ 104 293T-ACE2 cells (provided by Dr. Jesse Bloom, Fred Hutchinson Cancer Research Center) were seeded per well on poly-L-Lysine-coated 96-well plates (Corning) 18 hours before infection. 293T-ACE2suggested: RRID:CVCL_YZ65)Software and Algorithms Sentences Resources Codon optimization of transgene cDNA sequences was performed using the GeneArt GeneOptimizer software (Thermo Fisher Scientific) GeneArt GeneOptimizersuggested: NoneTZM-bl reporter cells (NIH AIDS Reagents Program) were added, and luminescence was measured after 48 hours. AIDS Reagents Programsuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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