SARS-CoV-2 multi-variant rapid detector based on graphene transistor functionalized with an engineered dimeric ACE2 receptor

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1. Version published to 10.1016/j.nantod.2022.101729

   Feb 1, 2023
2. 

   ScreenIT

   Oct 6, 2021

   SciScore for 10.1101/2021.10.02.21264210: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	Consent: Ethics approval: All subject provided written consent to be used for research purposes. IRB: The Ethics Committee of Region Marche (CERM) approved the study on 17/12/20.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   The samples were then subjected to SDS–PAGE and analysed by western blotting using an anti-Histidine antibody (Thermo Scientific) to detect Spike and ACE2, an anti-rabbit antibody (Santa Cruz Biotechnology) to detect Ab-CR3022 and an anti-ACE2 antibody (EMP Millipore Corp) to detect ACE2-Fc by chemiluminescent …

   SciScore for 10.1101/2021.10.02.21264210: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	Consent: Ethics approval: All subject provided written consent to be used for research purposes. IRB: The Ethics Committee of Region Marche (CERM) approved the study on 17/12/20.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   The samples were then subjected to SDS–PAGE and analysed by western blotting using an anti-Histidine antibody (Thermo Scientific) to detect Spike and ACE2, an anti-rabbit antibody (Santa Cruz Biotechnology) to detect Ab-CR3022 and an anti-ACE2 antibody (EMP Millipore Corp) to detect ACE2-Fc by chemiluminescent revelation39.	anti-Histidine suggested: None anti-rabbit suggested: None anti-ACE2 suggested: None
   The same protocol, using empty beads (without Spike), was performed as a negative control for each system. gFET functionalization: ACE-Fc, ACE2 and anti-Spike antibody were covalently immobilized over the fabricated gFET chip (Graphenea gFET-S20) through PBASE.	anti-Spike suggested: (Imported from the IEDB Cat# s20, RRID:AB_2847993)
   Experimental Models: Cell Lines
   Sentences	Resources
   Purification of Trimeric Spike protein from FreeStyle HEK293-F cells: The plasmid for expression of the SARS-CoV-2 prefusion-stabilized Spike ectodomain in HEK293-F cells (Thermo Fisher) was a generous gift from the McLellan laboratory at the University of Texas at Austin36.	HEK293-F suggested: RRID:CVCL_6642)
   Software and Algorithms
   Sentences	Resources
   After glycine treatment, samples were rinsed with PBS (pH 7.4, 1X), DI water and dried with N2. gFET characterization using Raman and AFM: AFM measurements were performed with a SOLVER PRO from NT-MDT, RMS was evaluated by using Nova Px software.	SOLVER suggested: (Solver, RRID:SCR_008510)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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3. 

   Version published to 10.1101/2021.10.02.21264210 on medRxiv

   Oct 4, 2021