Remdesivir is a delayed translocation inhibitor of SARS-CoV-2 replication
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
Article activity feed
-
-
SciScore for 10.1101/2020.12.14.422718: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Peaks were integrated with GeneMapper 5 software (ThermoFisher). GeneMappersuggested: (GeneMapper , RRID:SCR_014290)Images were recorded with SerialEM (Mastronarde, 2005), with a pixel size of 1.1Å over a defocus range of −1.5 to −2.5 μm. SerialEMsuggested: (SerialEM, RRID:SCR_017293)CTF correction, motion correction and particle picking were performed in real-time using WARP, resulting in 2,340,544 particles, which were uploaded to cryoSPARC v2 (Punjani et al., 2017). WARPsuggested…SciScore for 10.1101/2020.12.14.422718: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Peaks were integrated with GeneMapper 5 software (ThermoFisher). GeneMappersuggested: (GeneMapper , RRID:SCR_014290)Images were recorded with SerialEM (Mastronarde, 2005), with a pixel size of 1.1Å over a defocus range of −1.5 to −2.5 μm. SerialEMsuggested: (SerialEM, RRID:SCR_017293)CTF correction, motion correction and particle picking were performed in real-time using WARP, resulting in 2,340,544 particles, which were uploaded to cryoSPARC v2 (Punjani et al., 2017). WARPsuggested: (Warp, RRID:SCR_018071)Particles were subjected to multiple rounds of 3D classification in cryoSPARC, and a final set of 116,748 particles was refined to a global resolution of 3.89 Å based on the 0.143 FSC criterion. cryoSPARCsuggested: (cryoSPARC, RRID:SCR_016501)Non-proteinaceous molecules (i.e. RNA, ligands) were removed, and the template:primer duplex was built de novo in Coot (Emsley and Cowtan, 2004). Cootsuggested: (Coot, RRID:SCR_014222)Restraints for RMP were generated using eLBOW (Moriarty et al., 2009)), and structures were subjected to real-space refinement using Phenix (which resulted in a map-to-model FSC of 4.0Å at the 0.5 threshold) (Afonine et al., 2018). Phenixsuggested: (Phenix, RRID:SCR_014224)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on page 12. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
-
