Omicron neutralizing antibody response following booster vaccination compared with breakthrough infection

  1. Marcel E. Curlin
  2. Timothy A. Bates
  3. Gaelen Guzman
  4. Devin Schoen
  5. Savannah K. McBride
  6. Samuel D. Carpenter
  7. Fikadu G. Tafesse

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Abstract

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  1. Version published to 10.1016/j.medj.2022.09.001
  2. ScreenIT

    SciScore for 10.1101/2022.04.11.22273694: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    EthicsField Sample Permit: Fixation, development, and counting of FRNT plates was carried out as described in SARS-CoV-2 growth and titration.
    IRB: Study approval: This study was conducted with approval of the Oregon Health and Sciences University Institutional review board (IRB# 00022511).
    Consent: All participants were enrolled following written informed consent.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Experimental Models: Cell Lines
    SentencesResources
    Bead-serum mixtures were then incubated with 20,000 THP-1 cells in a final volume of 100 uL overnight in a tissue culture incubator.
    THP-1
    suggested: None
    3 Sub-confluent Vero E6 cells (CRL-1586) grown in Dulbecco’s Modified Eagle Medium (DMEM), 10% fetal bovine serum (FBS), 1% nonessential amino acids, 1% penicillin-streptomycin (complete media) were infected at an MOI of 0.05 in a minimal volume (0.01 mL/cm2) of Opti-MEM + 2% FBS (dilution media) for 1 hour at TCC then 0.1 mL/cm2 additional complete media was added and incubated until at least 20% cytopathic effect (CPE) was observed, typically 72-96 hours.
    Vero E6
    suggested: None
    Software and Algorithms
    SentencesResources
    Aggregated EC50 and FRNT50 values were analyzed in Graphpad Prism (9.3.1).
    Graphpad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2022.04.11.22273694v1 on medRxiv