Reduced pathogenicity of the SARS-CoV-2 omicron variant in hamsters
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
Article activity feed
-
-
SciScore for 10.1101/2022.01.02.474743: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: All animal studies were conducted in compliance with all relevant local, state, and federal regulations and were approved by the BIOQUAL Institutional Animal Care and Use Committee.
IACUC: All animal studies were conducted in compliance with all relevant local, state, and federal regulations and were approved by the BIOQUAL Institutional Animal Care and Use Committee.Sex as a biological variable Study design: Eight week old female and male golden Syrian hamsters (Envigo) were infected with SARS-CoV-2 in a volume of 100 µl (50 µl/nostril) by the intranasal route. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Tab…
SciScore for 10.1101/2022.01.02.474743: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: All animal studies were conducted in compliance with all relevant local, state, and federal regulations and were approved by the BIOQUAL Institutional Animal Care and Use Committee.
IACUC: All animal studies were conducted in compliance with all relevant local, state, and federal regulations and were approved by the BIOQUAL Institutional Animal Care and Use Committee.Sex as a biological variable Study design: Eight week old female and male golden Syrian hamsters (Envigo) were infected with SARS-CoV-2 in a volume of 100 µl (50 µl/nostril) by the intranasal route. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Omicron Stock 2 had a titer of 2.3×108 TCID50/ml and 3.8×106 PFU/ml in VeroE6-TMPRSS2 cells (EPI_ISL_7263803; Yoshihiro Kawaoka, University of Wisconsin). VeroE6-TMPRSS2suggested: NoneRecombinant DNA Sentences Resources 9 The gene fragments were subsequently cloned into a pcDNA3.1+ expression plasmid using restriction site cloning (Integrated DNA Technologies). pcDNA3.1+suggested: RRID:Addgene_117272)Software and Algorithms Sentences Resources Statistical analyses: Analysis of virologic and body weight data was performed using GraphPad Prism 9.1.2 (GraphPad Software). GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
-
