A monocyte/dendritic cell molecular signature of SARS-CoV-2-related multisystem inflammatory syndrome in children with severe myocarditis
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SciScore for 10.1101/2021.02.23.432486: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Our study has several limitations, including the relatively low number of cases in each group, the lack of a comparison with asymptomatic or mildly symptomatic non-hospitalized children positive for SARS-CoV-2 and a longitudinal study of children with “classic” KD enrolled before the COVID-19 pandemic. All our cellular data were …
SciScore for 10.1101/2021.02.23.432486: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Our study has several limitations, including the relatively low number of cases in each group, the lack of a comparison with asymptomatic or mildly symptomatic non-hospitalized children positive for SARS-CoV-2 and a longitudinal study of children with “classic” KD enrolled before the COVID-19 pandemic. All our cellular data were generated from frozen peripheral mononuclear cells, which does not allow a direct assessment of neutrophils. A parallel analysis of PMN (polymorphonuclear leukocytes) will be required. Endothelial and myocardiac cells are at least targets of the disease but may also contribute to the pathophysiology as described above. Also, additional data supporting gene expression findings will be necessary in future studies. Nevertheless, our study provides further in-depth molecular analysis of MIS-C with severe myocarditis. These severe forms were found to be associated with an excessive activation of the TNF-α, NF-κB signaling axis and poor response to type-I and type-II interferons in monocytes and DCs, secretion of cytokines promoting angiogenesis, chemotaxis and potential migration of activated myeloid cells and neutrophils in the myocardiac tissue. This may help to identify potential new clinical biomarkers and open new therapeutic strategies, including drugs targeting TNF-α or NF-κB pathways.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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