Multiplexed biosensor for point-of-care COVID-19 monitoring: CRISPR-powered unamplified RNA diagnostics and protein-based therapeutic drug management
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SciScore for 10.1101/2022.03.30.22271928: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Antibodies Sentences Resources As the glucose oxidase (GOx)-conjugated (see Supporting Information) monoclonal anti-6-FAM antibody (SAB4600050-125UL, Merck, Germany) will now only bind to the 6-FAM label on the 5’ end of uncleaved reRNA, the signal obtained in the amperometric readout is highly specific and inversely proportional to the target concentration within the sample solution. β-lactam Detection: Functionalization of the immobilization area is accomplished by incubation with penicillin binding protein 3 (PBP-337; 250 µg ml−1 in PBS (10 mM; … SciScore for 10.1101/2022.03.30.22271928: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Antibodies Sentences Resources As the glucose oxidase (GOx)-conjugated (see Supporting Information) monoclonal anti-6-FAM antibody (SAB4600050-125UL, Merck, Germany) will now only bind to the 6-FAM label on the 5’ end of uncleaved reRNA, the signal obtained in the amperometric readout is highly specific and inversely proportional to the target concentration within the sample solution. β-lactam Detection: Functionalization of the immobilization area is accomplished by incubation with penicillin binding protein 3 (PBP-337; 250 µg ml−1 in PBS (10 mM; pH 7.4), expressed via in-house production) for 1 hour, followed by 30 minutes of blocking with biotin-free casein (85R-108, Fitzgerald, USA). anti-6-FAMsuggested: NoneSAB4600050-125ULsuggested: Nonepenicillin binding protein 3suggested: Nonecasein ( 85R-108suggested: NoneSoftware and Algorithms Sentences Resources The protein concentration is determined by use of the Bradford method (Bio-Rad laboratories, USA) with bovine serum albumin (Carl Roth, Germany) as standard and the concentration of the pure protein is determined by analyzing the SDS gels with ImageJ. ImageJsuggested: (ImageJ, RRID:SCR_003070)Clinical Samples and RNA-Extraction: Patient samples were provided by the Medical Center – University of Freiburg and lysed in the Alinity Lysis Solution (09N20-001, Abbott, USA). Abbottsuggested: (Abbott, RRID:SCR_010477)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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