Nanotraps for the containment and clearance of SARS-CoV-2

Article activity feed

1. Version published to 10.1016/j.matt.2021.04.005

   Jun 1, 2021
2. 

   ScreenIT

   Mar 1, 2021

   SciScore for 10.1101/2021.02.01.428871: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: The animal study protocols were approved by the Institutional Animal Care and Use Committee (IACUC) of University of Chicago. IRB: This study was deemed exempt by the University of Chicago Institutional Review Board (IRB19-1942)
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	To evaluate the safety of the Nanotraps, 2 male and 2 female 6-10-week-old C57BL/6NHsd mice were intratracheally administered with 10 mg/kg Nanotrap-ACE2 in 50 μL PBS.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   The pellet was resuspended in 100 μL PBS and incubated with biotinylated ACE2 (Bioss …

   SciScore for 10.1101/2021.02.01.428871: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: The animal study protocols were approved by the Institutional Animal Care and Use Committee (IACUC) of University of Chicago. IRB: This study was deemed exempt by the University of Chicago Institutional Review Board (IRB19-1942)
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	To evaluate the safety of the Nanotraps, 2 male and 2 female 6-10-week-old C57BL/6NHsd mice were intratracheally administered with 10 mg/kg Nanotrap-ACE2 in 50 μL PBS.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   The pellet was resuspended in 100 μL PBS and incubated with biotinylated ACE2 (Bioss Antibodies) for 30 minutes on ice.	ACE2 suggested: None
   AF488-labeled anti-ACE2 antibody (Santa Cruz Biotechnology) was added to the Nanotrap-ACE2 for 30 minutes on ice and centrifuged at 5,000×g for 10 min, the pellet was washed with PBS for 3 times.	AF488-labeled suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   The differentiated THP-1 cells were harvested as dTHP-1 macrophages and maintained in RPMI supplemented with 10% FBS and 1% Penicillin-Streptomycin.	THP-1 suggested: None
   After 24 hours, 3×107 FFU VSVdG*G-GFP virus was added to the HEK293T cells and incubated for another 48 hours.	HEK293T suggested: None
   Coculture was carried out in a macrophage to A549 cell ratio of 1:5. 4×104 A549 cells were seeded in an 18-well microslide (Vivid) overnight and 8×103 dTHP-1 macrophages were added onto the A549 cells for another 6 hours.	A549 suggested: None
   In vitro cytotoxicity assay: A549 or HEK293T-ACE2 cells (both maintained in DMEM supplemented with 10% FBS and 1% Penicillin-Streptomycin) were seed in a 96-well plate at a density of 1×104 cells/well in 100 μL of culture medium overnight.	HEK293T-ACE2 suggested: None
   African green monkey kidney (Vero E6) cells were maintained in DMEM supplemented with 10% FBS and 1% Penicillin-Streptomycin.	Vero E6 suggested: None
   Experimental Models: Organisms/Strains
   Sentences	Resources
   To evaluate the safety of the Nanotraps, 2 male and 2 female 6-10-week-old C57BL/6NHsd mice were intratracheally administered with 10 mg/kg Nanotrap-ACE2 in 50 μL PBS.	C57BL/6NHsd suggested: None
   Software and Algorithms
   Sentences	Resources
   Line scans were performed in Fiji.	Fiji suggested: (Fiji, RRID:SCR_002285)
   The data were further analyzed by FlowJo (BD) and Prism (Graphpad) softwares.	FlowJo suggested: (FlowJo, RRID:SCR_008520) Graphpad suggested: (GraphPad, RRID:SCR_000306)
   Image reconstruction videos were made in Imaris (Bitplane).	Imaris suggested: (Imaris, RRID:SCR_007370)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
3. 

   Version published to 10.1101/2021.02.01.428871v1 on bioRxiv

   Feb 1, 2021