Nanotraps for the containment and clearance of SARS-CoV-2
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SciScore for 10.1101/2021.02.01.428871: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: The animal study protocols were approved by the Institutional Animal Care and Use Committee (IACUC) of University of Chicago.
IRB: This study was deemed exempt by the University of Chicago Institutional Review Board (IRB19-1942)Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable To evaluate the safety of the Nanotraps, 2 male and 2 female 6-10-week-old C57BL/6NHsd mice were intratracheally administered with 10 mg/kg Nanotrap-ACE2 in 50 μL PBS. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources The pellet was resuspended in 100 μL PBS and incubated with biotinylated ACE2 (Bioss … SciScore for 10.1101/2021.02.01.428871: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: The animal study protocols were approved by the Institutional Animal Care and Use Committee (IACUC) of University of Chicago.
IRB: This study was deemed exempt by the University of Chicago Institutional Review Board (IRB19-1942)Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable To evaluate the safety of the Nanotraps, 2 male and 2 female 6-10-week-old C57BL/6NHsd mice were intratracheally administered with 10 mg/kg Nanotrap-ACE2 in 50 μL PBS. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources The pellet was resuspended in 100 μL PBS and incubated with biotinylated ACE2 (Bioss Antibodies) for 30 minutes on ice. ACE2suggested: NoneAF488-labeled anti-ACE2 antibody (Santa Cruz Biotechnology) was added to the Nanotrap-ACE2 for 30 minutes on ice and centrifuged at 5,000×g for 10 min, the pellet was washed with PBS for 3 times. AF488-labeledsuggested: NoneExperimental Models: Cell Lines Sentences Resources The differentiated THP-1 cells were harvested as dTHP-1 macrophages and maintained in RPMI supplemented with 10% FBS and 1% Penicillin-Streptomycin. THP-1suggested: NoneAfter 24 hours, 3×107 FFU VSVdG*G-GFP virus was added to the HEK293T cells and incubated for another 48 hours. HEK293Tsuggested: NoneCoculture was carried out in a macrophage to A549 cell ratio of 1:5. 4×104 A549 cells were seeded in an 18-well microslide (Vivid) overnight and 8×103 dTHP-1 macrophages were added onto the A549 cells for another 6 hours. A549suggested: NoneIn vitro cytotoxicity assay: A549 or HEK293T-ACE2 cells (both maintained in DMEM supplemented with 10% FBS and 1% Penicillin-Streptomycin) were seed in a 96-well plate at a density of 1×104 cells/well in 100 μL of culture medium overnight. HEK293T-ACE2suggested: NoneAfrican green monkey kidney (Vero E6) cells were maintained in DMEM supplemented with 10% FBS and 1% Penicillin-Streptomycin. Vero E6suggested: NoneExperimental Models: Organisms/Strains Sentences Resources To evaluate the safety of the Nanotraps, 2 male and 2 female 6-10-week-old C57BL/6NHsd mice were intratracheally administered with 10 mg/kg Nanotrap-ACE2 in 50 μL PBS. C57BL/6NHsdsuggested: NoneSoftware and Algorithms Sentences Resources Line scans were performed in Fiji. Fijisuggested: (Fiji, RRID:SCR_002285)The data were further analyzed by FlowJo (BD) and Prism (Graphpad) softwares. FlowJosuggested: (FlowJo, RRID:SCR_008520)Graphpadsuggested: (GraphPad, RRID:SCR_000306)Image reconstruction videos were made in Imaris (Bitplane). Imarissuggested: (Imaris, RRID:SCR_007370)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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