Glycolytic inhibitor 2-deoxy-d-glucose attenuates SARS-CoV-2 multiplication in host cells and weakens the infective potential of progeny virions
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SciScore for 10.1101/2021.06.12.448175: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources A similar protocol was followed for establishing B.6 variant in Vero cells, at the BSL-3 facility, CSIR-CCMB, Hyderabad. Verosuggested: CLS Cat# 605372/p622_VERO, RRID:CVCL_0059)Estimation of glucose uptake: The qualitative and quantitative estimation of glucose uptake was performed using 2-NBDG (200μM) in Vero E6 cells. Vero E6suggested: RRID:CVCL_XD71)Similarly, for differential glucose uptake in Vero E6/Vero cells initially, Vero cells (0.025×106) were stained … SciScore for 10.1101/2021.06.12.448175: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources A similar protocol was followed for establishing B.6 variant in Vero cells, at the BSL-3 facility, CSIR-CCMB, Hyderabad. Verosuggested: CLS Cat# 605372/p622_VERO, RRID:CVCL_0059)Estimation of glucose uptake: The qualitative and quantitative estimation of glucose uptake was performed using 2-NBDG (200μM) in Vero E6 cells. Vero E6suggested: RRID:CVCL_XD71)Similarly, for differential glucose uptake in Vero E6/Vero cells initially, Vero cells (0.025×106) were stained with CellTracker™ Red CMPTX (Invitrogen) using (5μM; 30mins; 37°C) followed by normal growth medium replacement and co-culture with unstained Vero E6 cells (0.025×106). Vero E6/Verosuggested: NoneSoftware and Algorithms Sentences Resources For this purpose, the study employed algorithms from CELLSEGM and Image Processing toolbox in Matlab 2020a (Mathworks). Matlabsuggested: (MATLAB, RRID:SCR_001622)Densitometry was performed for each blot using Image J software and obtained values were normalized with respective loading control ß-actin, and the graph presented as relative fold change among the treatment groups. Image Jsuggested: (ImageJ, RRID:SCR_003070)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on page 28. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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