Detection of SARS-CoV-2 nucleocapsid antigen from serum can aid in timing of COVID-19 infection

  1. M.J. Ahava
  2. S. Kurkela
  3. S. Kuivanen
  4. M. Lappalainen
  5. H. Jarva
  6. A.J. Jääskeläinen

Reviewed by

Read the full article See related articles

Listed in

Log in to save this article

Abstract

No abstract available

Article activity feed

  1. Version published to 10.1016/j.jviromet.2022.114469
  2. ScreenIT

    SciScore for 10.1101/2021.01.08.20248771: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIRB: Research permit HUS/157/2020-44 (Helsinki University Hospital, Finland) was obtained from the local review board.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variableThe median age of the negative panel cases was 53 years (range 2-89); 45% were males (66/148).

    Table 2: Resources

    Antibodies
    SentencesResources
    Of the 155 specimens, 37 were sent for respiratory virus antibody testing; 32 samples were positive for anti-nuclear antibodies; 16 for phospholipase-A2-receptor antibodies; 8 for antineutrophil cytoplasmic (C-ANCA, P-ANCA and parallel C- and P-ANCA), and 4 for glomerular basement membrane antibodies.
    anti-nuclear antibodies; 16 for phospholipase-A2-receptor antibodies; 8 for antineutrophil cytoplasmic ( C-ANCA
    suggested: None
    Software and Algorithms
    SentencesResources
    They were also tested for SARS-CoV-2 IgG by both Abbott SARS-CoV-2 IgG (N antigen) and Euroimmun SARS-CoV-2 IgG (S1 antigen) according to manufacturer’s instructions.
    Abbott
    suggested: (Abbott, RRID:SCR_010477)
    Adjusted p-values for comparison of antigen and MNT result combinations were determined using Kruskal-Wallis test (GraphPad Prism 8.0.1).
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)
    The 95% Clopper-Pearson confidence intervals were calculated for sensitivity and specificity (IBM SPSS statistical program package, version 25).
    SPSS
    suggested: (SPSS, RRID:SCR_002865)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.01.08.20248771v1 on medRxiv