Evaluation of two RT-PCR techniques for SARS-CoV-2 RNA detection in serum for microbiological diagnosis
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SciScore for 10.1101/2020.11.15.20231795: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Eighty nine were admitted to the ICU (Intensive Care Unit) with a median age of 65 years (IQR 69-72) and 71.3% male sex; and 114 were admitted to the general ward with a median age of 64 years; (IQR 54.3-72) and 66.4% of them men. Table 2: Resources
Software and Algorithms Sentences Resources Statistical analysis was performed using SPSS 25.0 (IBM Corp., USA). SPSSsuggested: (SPSS, RRID:SCR_002865)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature …
SciScore for 10.1101/2020.11.15.20231795: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Eighty nine were admitted to the ICU (Intensive Care Unit) with a median age of 65 years (IQR 69-72) and 71.3% male sex; and 114 were admitted to the general ward with a median age of 64 years; (IQR 54.3-72) and 66.4% of them men. Table 2: Resources
Software and Algorithms Sentences Resources Statistical analysis was performed using SPSS 25.0 (IBM Corp., USA). SPSSsuggested: (SPSS, RRID:SCR_002865)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:TaqPath™ test is more suitable performing a small number of tests at a time meanwhile the cobas® test should be more useful when it is necessary to analyze high amounts of samples The present study has some limitations. First, the intrinsic analytical variability of PCR can have influenced in the results obtained. Another limitation is the type of sample used in this study. Although serum samples are accepted in the analysis of viraemias, the common samples used in most microbiology laboratories are plasma samples. In conclusion, this study shows that cobas® SARS-CoV-2 Test and TaqPath™ COVID-19 CE-IVD RT-PCR Kit could be useful in SARS-CoV-2 viraemia detection in serum of diagnosed COVID-19 patients. For a better test performance, considering the detection of at least one target obtained with any technique, and in particular with a Ct value under 37 with the TaqPath™ assay. A viraemia positive result may help in handling COVID-19 patients, due to the relationship between viraemia and bad prognosis and mortality recently reported (4, 15). In addition, these techniques are easy to incorporate in the Microbiology laboratory routine for SARS-CoV-2 diagnosis. Finally it could open new treatment strategies based on early COVID-19 treatment (15).
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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