The theory and practice of the viral dose in neutralization assay: Insights on SARS-CoV-2 “doublethink” effect
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SciScore for 10.1101/2020.10.16.342428: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources (ELISA): Specific anti-SARS-CoV-2 IgG antibodies were detected through a commercial ELISA kit (Euroimmun, Lübeck, Germany). anti-SARS-CoV-2 IgGsuggested: NoneAccording to the manufacturer, cross-reactions may occur with anti-SARS-CoV(−1) IgG antibodies, due to the close relationship between SARS-CoV(−1) and SARS-CoV-2, while cross-reactions with other human pathogenic CoVs (MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-HKU1, and HCoV-OC43) are excluded. anti-SARS-CoV…SciScore for 10.1101/2020.10.16.342428: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources (ELISA): Specific anti-SARS-CoV-2 IgG antibodies were detected through a commercial ELISA kit (Euroimmun, Lübeck, Germany). anti-SARS-CoV-2 IgGsuggested: NoneAccording to the manufacturer, cross-reactions may occur with anti-SARS-CoV(−1) IgG antibodies, due to the close relationship between SARS-CoV(−1) and SARS-CoV-2, while cross-reactions with other human pathogenic CoVs (MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-HKU1, and HCoV-OC43) are excluded. anti-SARS-CoV(−1) IgGsuggested: NoneSARS-CoV-2suggested: NoneHCoV-HKU1suggested: NoneExperimental Models: Cell Lines Sentences Resources The virus was titrated in serial 1 log dilutions (from 1 log to 11 log) to obtain a 50% tissue culture infective dose (TCID50) on 96-well culture plates of VERO and VERO E6 cells. VERO E6suggested: RRID:CVCL_XD71)According to the manufacturer, cross-reactions may occur with anti-SARS-CoV(−1) IgG antibodies, due to the close relationship between SARS-CoV(−1) and SARS-CoV-2, while cross-reactions with other human pathogenic CoVs (MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-HKU1, and HCoV-OC43) are excluded. HCoV-NL63suggested: RRID:CVCL_RW88)Software and Algorithms Sentences Resources Statistics analysis: Data analysis was performed using GraphPad Prism Version 5. GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
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