Does sampling saliva increase detection of SARS-CoV-2 by RT-PCR? Comparing saliva with oro-nasopharyngeal swabs
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SciScore for 10.1101/2020.07.26.20158618: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: All subjects provided informed consent, and the study was approved by the ethics committee of Umraniye Teaching and Research Hospital (B.10.1.THK.4.34.H.GP.0.01/167)Patients admitted to ICU, not giving consent to study, incapable of providing saliva sample and patients under age of 18 were excluded.
IRB: All subjects provided informed consent, and the study was approved by the ethics committee of Umraniye Teaching and Research Hospital (B.10.1.THK.4.34.H.GP.0.01/167)Patients admitted to ICU, not giving consent to study, incapable of providing saliva sample and patients under age of 18 were excluded.Randomization not detected. Blinding not detected. Power … SciScore for 10.1101/2020.07.26.20158618: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: All subjects provided informed consent, and the study was approved by the ethics committee of Umraniye Teaching and Research Hospital (B.10.1.THK.4.34.H.GP.0.01/167)Patients admitted to ICU, not giving consent to study, incapable of providing saliva sample and patients under age of 18 were excluded.
IRB: All subjects provided informed consent, and the study was approved by the ethics committee of Umraniye Teaching and Research Hospital (B.10.1.THK.4.34.H.GP.0.01/167)Patients admitted to ICU, not giving consent to study, incapable of providing saliva sample and patients under age of 18 were excluded.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Statistical Analysis: SPSS. SPSSsuggested: (SPSS, RRID:SCR_002865)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:The most important limitation of our study is that no study has been performed in asymptomatic individuals and mild cases that do not require hospitalization. Therefore, it was not possible to make a comment about whether saliva samples can be used in screening. In conclusion, our study shows that the saliva sample is not as sensitive as standard nasopharyngeal swabs in determining viral RNA and it does not improve detection rate in PCR negative patient group. However, in the later stage of the disease, RT-PCR test from saliva samples might help detecting deteriorating patients or determining the isolation period more effectively after treatment.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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SciScore for 10.1101/2020.07.26.20158618: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement All subjects provided informed consent, and the study was approved by the ethics committee of Umraniye Teaching and Research Hospital (B.10.1.THK.4.34.H.GP.0.01/167) Patients admitted to ICU, not giving consent to study, incapable of providing saliva sample and patients under age of 18 were excluded. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Statistical Analysis: SPSS. SPSSsuggested: (SPSS, RRID:SCR_002865)Resul…
SciScore for 10.1101/2020.07.26.20158618: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement All subjects provided informed consent, and the study was approved by the ethics committee of Umraniye Teaching and Research Hospital (B.10.1.THK.4.34.H.GP.0.01/167) Patients admitted to ICU, not giving consent to study, incapable of providing saliva sample and patients under age of 18 were excluded. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Statistical Analysis: SPSS. SPSSsuggested: (SPSS, RRID:SCR_002865)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:
The most important limitation of our study is that no study has been performed in asymptomatic individuals and mild cases that do not require hospitalization. Therefore, it was not possible to make a comment about whether saliva samples can be used in screening. In conclusion, our study shows that the saliva sample is not as sensitive as standard nasopharyngeal swabs in determining viral RNA and it does not improve detection rate in PCR negative patient group. However, in the later stage of the disease, RT-PCR test from saliva samples might help detecting deteriorating patients or determining the isolation period more effectively after treatment. Acknowledgements: We thank to Murat Kaya for technical assistance Conflict of Interest: None Funding: None
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
About SciScore
SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore is not a substitute for expert review. SciScore checks for the presence and correctness of RRIDs (research resource identifiers) in the manuscript, and detects sentences that appear to be missing RRIDs. SciScore also checks to make sure that rigor criteria are addressed by authors. It does this by detecting sentences that discuss criteria such as blinding or power analysis. SciScore does not guarantee that the rigor criteria that it detects are appropriate for the particular study. Instead it assists authors, editors, and reviewers by drawing attention to sections of the manuscript that contain or should contain various rigor criteria and key resources. For details on the results shown here, including references cited, please follow this link.
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SciScore for 10.1101/2020.07.26.20158618: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement All subjects provided informed consent, and the study was approved by the ethics committee of Umraniye Teaching and Research Hospital (B.10.1.THK.4.34.H.GP.0.01/167) Patients admitted to ICU, not giving consent to study, incapable of providing saliva sample and patients under age of 18 were excluded. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Statistical Analysis: SPSS. SPSSsuggested: (SPSS, SCR_002865)Data from additional tools added to each annotation on a …
SciScore for 10.1101/2020.07.26.20158618: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement All subjects provided informed consent, and the study was approved by the ethics committee of Umraniye Teaching and Research Hospital (B.10.1.THK.4.34.H.GP.0.01/167) Patients admitted to ICU, not giving consent to study, incapable of providing saliva sample and patients under age of 18 were excluded. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Statistical Analysis: SPSS. SPSSsuggested: (SPSS, SCR_002865)Data from additional tools added to each annotation on a weekly basis.
About SciScore
SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore is not a substitute for expert review. SciScore checks for the presence and correctness of RRIDs (research resource identifiers) in the manuscript, and detects sentences that appear to be missing RRIDs. SciScore also checks to make sure that rigor criteria are addressed by authors. It does this by detecting sentences that discuss criteria such as blinding or power analysis. SciScore does not guarantee that the rigor criteria that it detects are appropriate for the particular study. Instead it assists authors, editors, and reviewers by drawing attention to sections of the manuscript that contain or should contain various rigor criteria and key resources. For details on the results shown here, including references cited, please follow this link.
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