A fluorescence-based high throughput-screening assay for the SARS-CoV RNA synthesis complex
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Abstract
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SciScore for 10.1101/2020.07.07.192005: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources 2.5 IC50 determination based on the fluorescent Picogreen® assay: The compound’s concentration leading to a 50% inhibition of polymerase mediated RNA synthesis was determined in IC50 buffer (50 mM HEPES pH 8.0, 10 mM KCl, 2 mM MnCl2, 2 mM MgCl2, 10 mM DTT) containing 350 nM of Poly(A) template, 150 nM of nsp12 in complex with 1.5 µM nsp7L8 using 7 different concentrations of compound. Poly(Asuggested: NoneIC50 was determined from curve-fitting using Prism software. Prismsuggested: …SciScore for 10.1101/2020.07.07.192005: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources 2.5 IC50 determination based on the fluorescent Picogreen® assay: The compound’s concentration leading to a 50% inhibition of polymerase mediated RNA synthesis was determined in IC50 buffer (50 mM HEPES pH 8.0, 10 mM KCl, 2 mM MnCl2, 2 mM MgCl2, 10 mM DTT) containing 350 nM of Poly(A) template, 150 nM of nsp12 in complex with 1.5 µM nsp7L8 using 7 different concentrations of compound. Poly(Asuggested: NoneIC50 was determined from curve-fitting using Prism software. Prismsuggested: (PRISM, RRID:SCR_005375)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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