ACE2 and TMPRSS2 are expressed on the human ocular surface, suggesting susceptibility to SARS-CoV-2 infection
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SciScore for 10.1101/2020.05.09.086165: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Study protocols were approved by the Institutional Review Board at the Johns Hopkins School of Medicine, and study participants underwent informed consent by the treating surgeon. 2.2.
Consent: Study protocols were approved by the Institutional Review Board at the Johns Hopkins School of Medicine, and study participants underwent informed consent by the treating surgeon. 2.2.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Anti-ACE2 (ab108252, 1:1000) and anti-TMPRSS2 (ab92323, 1:1000) antibodies were purchased from Abcam. Anti-ACE2suggested: (Abcam …SciScore for 10.1101/2020.05.09.086165: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Study protocols were approved by the Institutional Review Board at the Johns Hopkins School of Medicine, and study participants underwent informed consent by the treating surgeon. 2.2.
Consent: Study protocols were approved by the Institutional Review Board at the Johns Hopkins School of Medicine, and study participants underwent informed consent by the treating surgeon. 2.2.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Anti-ACE2 (ab108252, 1:1000) and anti-TMPRSS2 (ab92323, 1:1000) antibodies were purchased from Abcam. Anti-ACE2suggested: (Abcam Cat# ab108252, RRID:AB_10864415)ab108252suggested: (Abcam Cat# ab108252, RRID:AB_10864415)anti-TMPRSS2suggested: (Abcam Cat# ab92323, RRID:AB_10585592)ab92323suggested: (Abcam Cat# ab92323, RRID:AB_10585592)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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SciScore for 10.1101/2020.05.09.086165: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Study protocols were approved by the Institutional Review Board at the Johns Hopkins School of Medicine , and study participants underwen informed consent by the treating surgeon . 2.2 . Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Total After blocking in 5 % normal goat serum diluted in PBS , the sections were incubated with anti-ACE2 antibody ( ab108252 , 1: 100 , Abcam , Cambridge , MA) , anti-TMPRSS2 antibody ( ab92323 , 1: 1000 , Abcam ) and the isotype- and concentration-matched normal IgG control ( ab172730 , Abcam ) overnigh … SciScore for 10.1101/2020.05.09.086165: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Study protocols were approved by the Institutional Review Board at the Johns Hopkins School of Medicine , and study participants underwen informed consent by the treating surgeon . 2.2 . Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Total After blocking in 5 % normal goat serum diluted in PBS , the sections were incubated with anti-ACE2 antibody ( ab108252 , 1: 100 , Abcam , Cambridge , MA) , anti-TMPRSS2 antibody ( ab92323 , 1: 1000 , Abcam ) and the isotype- and concentration-matched normal IgG control ( ab172730 , Abcam ) overnigh o at 4 C . anti-ACE2suggested: (Abcam Cat# ab108252, AB_10864415)For both corneal and lung sections , immunohistochemical staining with isotype IgG isotype antibody staining was performed , as negative controls ( Fig . 5D , G and J) . 3.6 . Grading of ACE2 and TMPRSS2 expression in epithelium on the ocular surface As an additional analysis , we graded the staining intensities of ACE2 and TMPRSS2 on the ocular surfa from 10 post-mortem eye specimens with particular attention to their expression in epithelium . isotype IgGsuggested: NoneParaffin- embedded human eye sections were processed for immunohistochemistry with rabbit anti-TMPRSS2 antibody. anti-TMPRSS2suggested: NoneParaffin-embedde human surgical conjunctival sections were analyzed by immunohistochemistry with rabbit anti-TMPRS antibody. anti-TMPRSsuggested: NoneSoftware and Algorithms Sentences Resources The susceptibility of the conjunctival to infection with SARS-CoV-2 provides further context for publish clinical studies . SARS-CoV-2suggested: (Sino Biological Cat# 40143-R019, AB_2827973)About SciScore
SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore is not a substitute for expert review. SciScore checks for the presence and correctness of RRIDs (research resource identifiers) in the manuscript, and detects sentences that appear to be missing RRIDs. SciScore also checks to make sure that rigor criteria are addressed by authors. It does this by detecting sentences that discuss criteria such as blinding or power analysis. SciScore does not guarantee that the rigor criteria that it detects are appropriate for the particular study. Instead it assists authors, editors, and reviewers by drawing attention to sections of the manuscript that contain or should contain various rigor criteria and key resources. For details on the results shown here, including references cited, please follow this link.
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