Multiplex PCR Assays for Identifying all Major Severe Acute Respiratory Syndrome Coronavirus 2 Variants
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SciScore for 10.1101/2021.10.28.21263107: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication Authentication: qRT-PCR screening was performed using 2 µL of purified RNA, TaqPath 1-step Multiplex Master Mix (No ROX) and an in-house validated multiplex assay. Table 2: Resources
Software and Algorithms Sentences Resources Synthetic RNA targets: RNA standards of the SARS-CoV-2 whole genome and variant spike proteins were provided by Exact Diagnostics through Bio-Rad Laboratories, Hercules, CA (catalog numbers COV019, COV019CE, COVA, COVB, COVE, COVG). Bio-Rad Laboratoriessuggested: (Bio-Rad Laboratories, RRID:SCR_008426)Primer Design: Primers … SciScore for 10.1101/2021.10.28.21263107: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication Authentication: qRT-PCR screening was performed using 2 µL of purified RNA, TaqPath 1-step Multiplex Master Mix (No ROX) and an in-house validated multiplex assay. Table 2: Resources
Software and Algorithms Sentences Resources Synthetic RNA targets: RNA standards of the SARS-CoV-2 whole genome and variant spike proteins were provided by Exact Diagnostics through Bio-Rad Laboratories, Hercules, CA (catalog numbers COV019, COV019CE, COVA, COVB, COVE, COVG). Bio-Rad Laboratoriessuggested: (Bio-Rad Laboratories, RRID:SCR_008426)Primer Design: Primers were designed by using the primer3 server13. primer3suggested: (Primer3, RRID:SCR_003139)The desired fractions were then ethanol precipitated and resuspended in water, and their concentration was determined by NanoDrop spectrophotometer. NanoDropsuggested: NoneMultiplex qRT-PCR with patient samples: Patient samples were tested in a similar manner to how we tested the assay’s sensitivity and specificity. 5 µL of each sample was combined with 15 µL each completed master mix using TaqPath and run as mentioned before. TaqPathsuggested: NoneResults from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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