The Mechanism of SARS-CoV-2 Nucleocapsid Protein Recognition by the Human 14-3-3 Proteins
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SciScore for 10.1101/2020.12.26.424450: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Protein expression and purification: The tagless SARS-CoV-2 N gene coding for Uniprot ID P0DTC9 protein sequence was commercially synthesized (GeneWiz) and cloned into pET-SUMO bacterial expression vectors using NdeI and HindIII restriction endonuclease sites. GeneWizsuggested: (GENEWIZ, RRID:SCR_003177)Protein concentration was determined by spectrophotometry at 280 nm on a N80 Nanophotometer (Implen, Munich, Germany) using sequence-specific extinction coefficients calculated … SciScore for 10.1101/2020.12.26.424450: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Protein expression and purification: The tagless SARS-CoV-2 N gene coding for Uniprot ID P0DTC9 protein sequence was commercially synthesized (GeneWiz) and cloned into pET-SUMO bacterial expression vectors using NdeI and HindIII restriction endonuclease sites. GeneWizsuggested: (GENEWIZ, RRID:SCR_003177)Protein concentration was determined by spectrophotometry at 280 nm on a N80 Nanophotometer (Implen, Munich, Germany) using sequence-specific extinction coefficients calculated using the ProtParam tool in ExPASy (see Supplementary table 5). ProtParamsuggested: (ProtParam Tool, RRID:SCR_018087)ExPASysuggested: NoneGraphing and fitting were performed in Origin 9.0 (OriginLab Corporation, Northampton, MA, USA) OriginLab Corporationsuggested: (Origin, RRID:SCR_014212)All processing was performed in ASTRA 8.0 software (Wyatt Technologies) taking dn/dc equal to 0.185 and using extinction coefficients listed in Supplementary table 5. ASTRAsuggested: (ASTRA, RRID:SCR_016255)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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