The ACE2-binding Interface of SARS-CoV-2 Spike Inherently Deflects Immune Recognition

Article activity feed

1. Version published to 10.1016/j.jmb.2020.166748

   Feb 1, 2021
2. 

   ScreenIT

   Nov 7, 2020

   SciScore for 10.1101/2020.11.03.365270: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	Consent: All patients gave written consent for this study and all samples were de-identified by following IRB #i20-00595.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   The staining with a secondary antibody (anti-human IgG (FC gamma specific)-Alexa488, Jackson Immunoresearch), diluted 1/800 in PBS containing 1 % BSA, was performed in a total volume of 25 µl for 30 minutes at room temperature with shaking.	anti-human IgG suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Establishment of ACE2-expressing …

   SciScore for 10.1101/2020.11.03.365270: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	Consent: All patients gave written consent for this study and all samples were de-identified by following IRB #i20-00595.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   The staining with a secondary antibody (anti-human IgG (FC gamma specific)-Alexa488, Jackson Immunoresearch), diluted 1/800 in PBS containing 1 % BSA, was performed in a total volume of 25 µl for 30 minutes at room temperature with shaking.	anti-human IgG suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Establishment of ACE2-expressing A549 cells: A549 cells were transfected with pLenti.ACE2 lentiviral vector that encodes ACE2 and puromycin resistance (Tada et al., Submitted).	A549 suggested: NCI-DTP Cat# A549, RRID:CVCL_0023)
   Cell-based binding assay: The ACE2-A549 cells were detached from the flask using the citric saline buffer containing 15 mM sodium citrate and 135 mM KCl.	ACE2-A549 suggested: None

   ---

   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

   ---

   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

   ---

   Results from TrialIdentifier: No clinical trial numbers were referenced.

   ---

   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

   ---

   Results from JetFighter: We did not find any issues relating to colormaps.

   ---

   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

   ---

   Read the original source
3. 

   Version published to 10.1101/2020.11.03.365270v1 on bioRxiv

   Nov 3, 2020