The ACE2-binding Interface of SARS-CoV-2 Spike Inherently Deflects Immune Recognition
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SciScore for 10.1101/2020.11.03.365270: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: All patients gave written consent for this study and all samples were de-identified by following IRB #i20-00595. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources The staining with a secondary antibody (anti-human IgG (FC gamma specific)-Alexa488, Jackson Immunoresearch), diluted 1/800 in PBS containing 1 % BSA, was performed in a total volume of 25 µl for 30 minutes at room temperature with shaking. anti-human IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources Establishment of ACE2-expressing … SciScore for 10.1101/2020.11.03.365270: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: All patients gave written consent for this study and all samples were de-identified by following IRB #i20-00595. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources The staining with a secondary antibody (anti-human IgG (FC gamma specific)-Alexa488, Jackson Immunoresearch), diluted 1/800 in PBS containing 1 % BSA, was performed in a total volume of 25 µl for 30 minutes at room temperature with shaking. anti-human IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources Establishment of ACE2-expressing A549 cells: A549 cells were transfected with pLenti.ACE2 lentiviral vector that encodes ACE2 and puromycin resistance (Tada et al., Submitted). A549suggested: NCI-DTP Cat# A549, RRID:CVCL_0023)Cell-based binding assay: The ACE2-A549 cells were detached from the flask using the citric saline buffer containing 15 mM sodium citrate and 135 mM KCl. ACE2-A549suggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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