Isolation of SARS-CoV-2 B.1.1.28.2 (P2) variant and pathogenicity comparison with D614G variant in hamster model

  1. Pragya Yadav
  2. Sreelekshmy Mohandas
  3. Prasad Sarkale
  4. Dimpal Nyayanit
  5. Anita Shete
  6. Rima Sahay
  7. Varsha Potdar
  8. Shrikant Baradkar
  9. Nivedita Gupta
  10. Gajanan Sapkal
  11. Priya Abraham
  12. Samiran Panda
  13. Balram Bhargava

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Abstract

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  1. Version published to 10.1016/j.jiph.2021.12.009
  2. ScreenIT

    SciScore for 10.1101/2021.05.24.445424: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    EthicsIACUC: 2.5 Ethics statement: All the animal experiments were performed with the approval of Institutional Animal Ethics Committee and as per the guidelines of CPCSEA, India. 2.6 Pathogenicity study in Syrian hamsters: To understand the pathogenicity of the isolate, we intranasally inoculated 104 TCID50 virus dose of Brazil P.2 (B.1.1.28.2) in 9 Syrian hamsters and observed them for 7 days for any disease and three hamsters from each group were sacrificed on day 3, 5 and 7 to study the organ viral load, antibody response and lung pathology.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    2.8 Enzyme-linked Immunosorbent Assay: The hamster serum samples collected on day 3, 5, 7 post virus inoculation were tested for IgG antibodies by hamster anti-SARS-CoV-2 IgG ELISA as described earlier [18].
    IgG
    suggested: None
    anti-SARS-CoV-2 IgG
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    Both the cases did not have any co-morbid conditions and were asymptomatic throughout the course of infection till recovery. 2.2 Cells and virus: Vero CCL81 cells grown in Minimum Essential Media (MEM) supplemented with 2% fetal bovine serum (FBS) were used.
    Vero CCL81
    suggested: None
    Software and Algorithms
    SentencesResources
    2.11 Data analysis: Graph pad Prism version 8.4.3 software was used for the analysis.
    Graph pad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: Thank you for sharing your data.

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.05.24.445424v1 on bioRxiv