Substantial immune response in Omicron infected breakthrough and unvaccinated individuals against SARS-CoV-2 variants of concern

  1. Pragya D. Yadav
  2. Gajanan N. Sapkal
  3. Rima R. Sahay
  4. Varsha A. Potdar
  5. Gururaj R. Deshpande
  6. Deepak Y. Patil
  7. Dimpal A. Nyayanit
  8. Anita M. Shete
  9. Jayanthi Shastri
  10. Pradip Awate
  11. Bharti Malhotra
  12. Priya Abraham

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Abstract

No abstract available

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  1. Version published to 10.1016/j.jinf.2022.02.005
  2. ScreenIT

    SciScore for 10.1101/2022.01.24.477043: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Ethicsnot detected.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Anti-SARS-CoV-2 IgG S1-RBD, N protein, Inactivated whole antigen ELISA: The samples were tested for anti-SARS-CoV-2 IgG antibodies against S1-RBD ELISA.
    Anti-SARS-CoV-2 IgG S1-RBD, N protein, Inactivated whole antigen ELISA
    suggested: None
    anti-SARS-CoV-2 IgG
    suggested: None
    S1-RBD ELISA.
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    Vero CCL-81 cell suspension (1.0 x 105 /mL/well) was added in 24-well tissue culture plates and was incubated for 16-24 hours at 37°C with 5% CO2 to obtain a confluent monolayer.
    Vero CCL-81
    suggested: None
    Software and Algorithms
    SentencesResources
    PRNT50 titre was calculated using a log probit regression analysis by SPSS (SPSS Inc., Chicago, IL).
    SPSS
    suggested: (SPSS, RRID:SCR_002865)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:
    The main limitation of this study are lesser participants in the unvaccinated group and the shorter window period post infection. This could be the important reason for the low immune response specifically in the unvaccinated individuals against Omicron. Our study suggest a 3-fold reduction in the NAb titres in BNT162b2 mRNA breakthrough individuals as compared with ChAdOx1 nCoV-19.The earlier studies from other groups demonstrated that immune response generated through natural infection or vaccination showed weaker immune response against Omicron [6–10]. Our study demonstrated that the individuals infected with Omicron have significant immune response which could neutralize not only the Omicron but also the other VOCs including most prevalent Delta variant. This suggest that the immune response induced by the Omicron could effectively neutralize the Delta variant making the re-infection with Delta less likely, thereby displacing the Delta as dominant strain. This emphasizes the need for the omicron specific vaccine strategy.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2022.01.24.477043v1 on bioRxiv