A highly effective reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for the rapid detection of SARS-CoV-2 infection
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SciScore for 10.1101/2020.06.30.20142935: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources This model was built in Excel using the add-in software Poptools20 (Supplementary information). Excelsuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:As with any diagnostic test, when it comes to the clinical application of Direct RT-LAMP, the …
SciScore for 10.1101/2020.06.30.20142935: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources This model was built in Excel using the add-in software Poptools20 (Supplementary information). Excelsuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:As with any diagnostic test, when it comes to the clinical application of Direct RT-LAMP, the pre-test probability of infection, based on clinical context and disease prevalence in the test subject or population, must be considered together with limitations of assay performance. We have provided a model, utilising published data on disease transmission from elsewhere, to illustrate the impact of pre-test probability on the positive predictive value (PPV) and negative predictive value (NPV) of Direct RT-LAMP in different scenarios. Depending on factors such as assay function (diagnosis vs screening), disease prevalence, patient group, setting and available resources, and their impact on PPV and NPV, further confirmation by a negative verification step may be considered desirable. It should be noted that the estimates of pre- and post-test probabilities of infection in this study were made only as an example of, and to help understand the use of the Direct RT-LAMP in practice. These estimates were based on crude approximations of the model’s parameter values (Supplementary information) and we encourage the readers who would like to use this model, to adjust the model and use parameter values that best suits the epidemiological situation of the country/region where the test would be applied. Rapid testing of symptomatic SARS-CoV-2 positive patients within healthcare facilities allows their rapid isolation or cohorting, significantly reducing onward transmission and improving bed...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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