Serological immunochromatographic approach in diagnosis with SARS-CoV-2 infected COVID-19 patients
This article has been Reviewed by the following groups
Discuss this preprint
Start a discussion What are Sciety discussions?Listed in
- Evaluated articles (ScreenIT)
Abstract
Article activity feed
-
-
SciScore for 10.1101/2020.03.13.20035428: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: This study was reviewed and approved by the Ethical Committee of Zhongnan Hospital of Wuhan University.
Consent: Written informed consent was waived by the Ethics Commission for emerging infectious diseases.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:However, the gold standard …
SciScore for 10.1101/2020.03.13.20035428: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: This study was reviewed and approved by the Ethical Committee of Zhongnan Hospital of Wuhan University.
Consent: Written informed consent was waived by the Ethics Commission for emerging infectious diseases.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:However, the gold standard also has its limitations. Based on more than 3,000 detected cases by real-time RT-PCR in the clinical laboratory of Zhongnan hospital, our previous review summarized several reasons for false-negative incidence, at both detection level and patients level (14). From detection aspects, the quality and sensitivity of detection kits and viral preservation solutions from different companies may all affect the detection accuracy and result in the false-negative possibility. Besides, it is indicated that, during the disease progress, the heavy infection at the nasopharyngeal area of early stage would possibly become negative and the lower respiratory tract may severely be infected at late stage. Thus, the nasopharyngeal swab may not be the best sampling site for all patients at various disease stage. Moreover, patients with persistent anti-viral medication (such as anti-HIV drugs) may decrease the viral loading at the undetectable level. Therefore, additional detection techniques are urgently needed in the supplement to the current diagnostic shortages for the nuclear acid-negative suspected cases, whom would otherwise be ruled out. In this study, we investigated the quick detection approach targeting viral IgM or IgG antibody, the colloidal gold-based immunochromatographic (ICG) strip assay, in comparison with real-time RT-PCR testing. The antibodies are produced and secreted by B lymphocytes of the adaptive immune system when the foreign pathogens invade...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a protocol registration statement.
-
