Artemisia annua L. extracts inhibit the in vitro replication of SARS-CoV-2 and two of its variants
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SciScore for 10.1101/2021.01.08.425825: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources The day prior to infection, Vero E6 and Calu-3 cells (ATCC HTB-55) were plated in black, clear-bottomed plates at 10,000 and 30,000 cells/well, respectively, in a final volume of 90 µl. Vero E6suggested: RRID:CVCL_XD71)Calu-3suggested: ATCC Cat# HTB-55, RRID:CVCL_0609)Software and Algorithms Sentences Resources Drug concentrations were log transformed and the concentration of drug(s) that inhibited virus by 50% (i.e., IC50), and the … SciScore for 10.1101/2021.01.08.425825: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources The day prior to infection, Vero E6 and Calu-3 cells (ATCC HTB-55) were plated in black, clear-bottomed plates at 10,000 and 30,000 cells/well, respectively, in a final volume of 90 µl. Vero E6suggested: RRID:CVCL_XD71)Calu-3suggested: ATCC Cat# HTB-55, RRID:CVCL_0609)Software and Algorithms Sentences Resources Drug concentrations were log transformed and the concentration of drug(s) that inhibited virus by 50% (i.e., IC50), and the concentration of drug(s) that killed 50% of cells (i.e., CC50), were determined via nonlinear logistic regressions of log(inhibitor) versus response-variable dose-response functions (four parameters) constrained to a zero-bottom asymptote by statistical analysis using GraphPad Prism 9 (GraphPad Software, Inc.) as described by Hulseberg et al. ( 2019). GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Statistical significance of artemisinin and total flavonoid content in hot water extracts was calculated via ANOVA using GraphPad Prism V8.0.2. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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SciScore for 10.1101/2021.01.08.425825: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Indicated dilutions of the drug were incubated for 1 h in wells of well tissue culture plates containing a monolayer of Vero E6 cells seeded the day before at 20,000 cells/well. Vero E6suggested: NoneThe day prior to infection, Vero E6 and Calu-3 cells (ATCC HTB-55) were plated in black, clear-bottomed plates at … SciScore for 10.1101/2021.01.08.425825: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Indicated dilutions of the drug were incubated for 1 h in wells of well tissue culture plates containing a monolayer of Vero E6 cells seeded the day before at 20,000 cells/well. Vero E6suggested: NoneThe day prior to infection, Vero E6 and Calu-3 cells (ATCC HTB-55) were plated in black, clear-bottomed plates at 10,000 and 30,000 cells/well, respectively, in a final volume of 90 µl. Calu-3suggested: ATCC Cat# HTB-55, RRID:CVCL_0609)Software and Algorithms Sentences Resources Drug concentrations were log transformed and the concentration of drug(s) that inhibited virus by 50% ( that killed 50% of cells ( log(inhibitor) versus response-variable dose-response functions (four parameters) constrained to a zero-bottom asymptote by statistical analysis using GraphPad Prism 9 (GraphPad Software, Inc.) as described by Hulseberg et al. ( 2019). GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)IC50 and IC90 values were calculated using GraphPad Prism V8.0. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
About SciScore
SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.
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