Comparative performance of multiplex salivary and commercially available serologic assays to detect SARS-CoV-2 IgG and neutralization titers
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SciScore for 10.1101/2021.01.28.21250717: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Ethics statement: This study used stored samples and data from two parent studies that were approved by The Johns Hopkins University School of Medicine Institutional Review Board.
Consent: All samples were de-identified prior to laboratory testing, and all participants provided informed consent.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected …SciScore for 10.1101/2021.01.28.21250717: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Ethics statement: This study used stored samples and data from two parent studies that were approved by The Johns Hopkins University School of Medicine Institutional Review Board.
Consent: All samples were de-identified prior to laboratory testing, and all participants provided informed consent.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:This study has several limitations. First, the sample size was relatively small and the cross-sectional design precluded evaluation of antibody dynamics over time, particularly given the relatively short period during which sample collection was undertaken (i.e. relative to symptom resolution). Nonetheless, this is consistent with most CCP collections and study populations, which provided insight into SARS-CoV-2 immunopathogenesis and screening options(3, 14). Second, the study population was primarily focused around Baltimore, MD and Washington DC, thus potentially limiting generalizability of the findings. Third, the Ortho Vitros EIA was validated for serum rather than plasma; while we do not believe that this impacted the results, it is a departure from the manufacturer’s instructions. Finally, a different saliva collection device (OraSure, Bethlehem, PA, USA) was used to establish the negative threshold for the multiplex SARS-CoV-2 IgG assay (Oracol+ S14, Malvern Medical Developments, Worcester, UK). A larger study is recommended to test reproducibility of the findings following the manufacturer instructions for the assay kits and using the same saliva collection devices. Nonetheless, the study employed a robust multiplex saliva assay approach to detect antibodies against SARS-CoV-2, demonstrating favorable performance both against several commercial EIAs, including the Ortho Vitros, as well as formal viral neutralization. When applied to saliva, the highly-adaptable mult...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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