Extraction-free RT-LAMP to detect SARS-CoV-2 is less sensitive but highly specific compared to standard RT-PCR in 101 samples
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SciScore for 10.1101/2020.12.07.20239558: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: All study procedures were reviewed and approved by the Biomedical Research Ethics Board, University of Manitoba. Randomization not detected. Blinding Sample processing and heat treatment: After assigning blinded sample identifiers to reduce potential bias in interpretation, samples were thawed and briefly spun down in a mini-centrifuge to collect cells and debris. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecog…SciScore for 10.1101/2020.12.07.20239558: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: All study procedures were reviewed and approved by the Biomedical Research Ethics Board, University of Manitoba. Randomization not detected. Blinding Sample processing and heat treatment: After assigning blinded sample identifiers to reduce potential bias in interpretation, samples were thawed and briefly spun down in a mini-centrifuge to collect cells and debris. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Despite these limitations, all tests applied on a wider scale, with proper messaging about the limited significance of negative results, could be highly useful if strong positives are identified and acted upon more quickly 25. For example, several jurisdictions are exploring use of PanBio Ag Rapid Test Device to identify positives early, assuming that negative results will need additional testing in order to be confirmed 26-28. Molecular diagnostic techniques that use raw or minimally processed sample are fastest, but less sensitive, since the background chemistry of a raw sample may introduce uncontrolled variability 18,29. Potential inhibitors or other contaminants may reduce the reliability of both positive and negative results. This concern is somewhat lessened in the context of the current report, which we have confirmed is highly specific and can amplify target molecules even in highly diluted samples. However, the current assay’s limit of detection (currently 1,000 copies/μl, similar to other studies 18) does decrease the likelihood of detecting a person with low viral load. In contrast, Sherlock Biosciences claims on its website (www.sherlock.bio) that its LAMP-based assay can detect 7 copies per μl VTM or up to 150 times less than this report. Is the risk of missing someone with a low viral load greater than making a person with a high viral load wait days to find out they are positive? In all cases, expectations about what a negative test result actually means must ...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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