Multicenter comparison of the Cobas 6800 system with the RealStar RT-PCR kit for the detection of SARS-CoV-2
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SciScore for 10.1101/2020.06.29.179184: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Statistical analysis: Statistical analyses were performed on GraphPad Prism version 6.0. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:This is a limitation of our study as we did not assessed …
SciScore for 10.1101/2020.06.29.179184: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Statistical analysis: Statistical analyses were performed on GraphPad Prism version 6.0. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:This is a limitation of our study as we did not assessed comparatively the limit of detection of the two methods but the reliability of their Ct values among COBAS 6800 positive samples, excluding those that could be negative with COBAS 6800 and positive with RealStar in this range of low viral loads. Our work highlights the impact of the extraction system on the sensitivity of the RealStar assay. Overall, we demonstrated the good performances and concordance between the two assays, at least for viral loads above the detection limit of both assays. This concordance allows to reliably compare Ct values obtained from both methods. However, the variations observed between the Ct values of the two assays, evaluated here as about 3.5 additional Ct with the Cobas 6800 assay, has to be taken into account for Ct values follow-up done for the most severe patients in case of successive use of the two methods, depending of reagent and analyser availability.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
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