Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccine
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SciScore for 10.1101/2022.01.31.478507: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable 34 Female Balb/cJ (5-10 weeks old, Jackson Labs) were euthanized with CO2. Randomization not detected. Blinding not detected. Power Analysis The second emulsion was sonicated at 65% power for 5 min at RT. Table 2: Resources
Antibodies Sentences Resources A secondary biotinylated anti-mouse IgA, total IgG, IgG1, or IgG2a antibody (SouthernBiotech) was diluted 5,000-fold in 5-fold diluted PBSTBA and was added to plates for 2 h at RT. A secondary biotinylated anti-mouse IgA, total IgG, IgG1, or IgG2a antibody (SouthernBiotech)suggested: Noneanti-mouse IgA, total IgGsuggested: NoneIgG1suggested: NoneIgG2asuggested: NoneExperimental Models: Organisms/Strains Sentences Re… SciScore for 10.1101/2022.01.31.478507: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable 34 Female Balb/cJ (5-10 weeks old, Jackson Labs) were euthanized with CO2. Randomization not detected. Blinding not detected. Power Analysis The second emulsion was sonicated at 65% power for 5 min at RT. Table 2: Resources
Antibodies Sentences Resources A secondary biotinylated anti-mouse IgA, total IgG, IgG1, or IgG2a antibody (SouthernBiotech) was diluted 5,000-fold in 5-fold diluted PBSTBA and was added to plates for 2 h at RT. A secondary biotinylated anti-mouse IgA, total IgG, IgG1, or IgG2a antibody (SouthernBiotech)suggested: Noneanti-mouse IgA, total IgGsuggested: NoneIgG1suggested: NoneIgG2asuggested: NoneExperimental Models: Organisms/Strains Sentences Resources To isolate T cells, spleens from female Balb/cJ mice were dissociated in 2 mg/mL Collagenase D (Sigma-Aldrich, Cat# 11088882001) in Opti-MEM media (Gibco, Cat# 11058021) and filtered through a 40 μm cell strainer. Balb/cJsuggested: NoneFemale BALB/c mice were intramuscularly injected with adjuvant-loaded NPs (4 mg/mouse) and variable doses of stabilized SARS-CoV-2 spike glycoprotein (BEI Resources, Cat# NR-52397) into the left and right anterior tibialis anterior muscles (50 μL NPs in saline per injection) on day 0 (prime) and day 28 (boost). BALB/csuggested: NoneSoftware and Algorithms Sentences Resources MPLA loading estimation via GC-MS, LC-MS, and surrogate fluorometry has been previously described.28 PUUC and CpG loading was quantified by supernatant measurement of unbound RNA or DNA, respectively, with the Nucleic Acid Quantification workflow on a Synergy HT plate reader (BioTek) with Gen5 software. Gen5suggested: (Gen5, RRID:SCR_017317)FlowJo Software (BD) was used to generate t-distributed stochastic neighbor embedding (t-SNE) plots. FlowJosuggested: (FlowJo, RRID:SCR_008520)Statistical analyses were performed with GraphPad Prism 9. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)For antibody quantification with ELISA, area under the curve (AUC) across fold dilutions was computed with the AUC function on GraphPad as previously reported. GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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