Rapid, high-yield production of full-length SARS-CoV-2 spike ectodomain by transient gene expression in CHO cells
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SciScore for 10.1101/2020.09.08.286732: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Protein-coding DNA sequences were codon-optimized for CHO cells, synthesized and cloned into the pTT5™ vector [18] by Genscript. CHOsuggested: CLS Cat# 603479/p746_CHO, RRID:CVCL_0213)2.2 Expression platforms: Transfection and post-transfection culture of CHO-3E7 and 293-6E cells in FreeStyle F17 media were performed as described previously [19, 20]. 293-6Esuggested: RRID:CVCL_HF20)The method for transient expression in CHOBRI/rcTA … SciScore for 10.1101/2020.09.08.286732: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Protein-coding DNA sequences were codon-optimized for CHO cells, synthesized and cloned into the pTT5™ vector [18] by Genscript. CHOsuggested: CLS Cat# 603479/p746_CHO, RRID:CVCL_0213)2.2 Expression platforms: Transfection and post-transfection culture of CHO-3E7 and 293-6E cells in FreeStyle F17 media were performed as described previously [19, 20]. 293-6Esuggested: RRID:CVCL_HF20)The method for transient expression in CHOBRI/rcTA cells [21] is based on a published method for high-density transfection of CHO-3E7 cells [19], with several modifications. CHOBRI/rcTAsuggested: NoneCHO-3E7suggested: RRID:CVCL_JY74)Software and Algorithms Sentences Resources Weighted average molecular mass (MMALS) was calculated in ASTRA 6.1 software (Wyatt) using a protein concentration determined from the refractive index signal with a dn/dc value of 0.185. ASTRAsuggested: (ASTRA, RRID:SCR_016255)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
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