Rapid, high-yield production of full-length SARS-CoV-2 spike ectodomain by transient gene expression in CHO cells

  1. Matthew Stuible
  2. Christian Gervais
  3. Simon Lord-Dufour
  4. Sylvie Perret
  5. Denis L’Abbé
  6. Joseph Schrag
  7. Gilles St-Laurent
  8. Yves Durocher

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Abstract

No abstract available

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  1. Version published to 10.1016/j.jbiotec.2020.12.005
  2. ScreenIT

    SciScore for 10.1101/2020.09.08.286732: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Experimental Models: Cell Lines
    SentencesResources
    Protein-coding DNA sequences were codon-optimized for CHO cells, synthesized and cloned into the pTT5™ vector [18] by Genscript.
    CHO
    suggested: CLS Cat# 603479/p746_CHO, RRID:CVCL_0213)
    2.2 Expression platforms: Transfection and post-transfection culture of CHO-3E7 and 293-6E cells in FreeStyle F17 media were performed as described previously [19, 20].
    293-6E
    suggested: RRID:CVCL_HF20)
    The method for transient expression in CHOBRI/rcTA cells [21] is based on a published method for high-density transfection of CHO-3E7 cells [19], with several modifications.
    CHOBRI/rcTA
    suggested: None
    CHO-3E7
    suggested: RRID:CVCL_JY74)
    Software and Algorithms
    SentencesResources
    Weighted average molecular mass (MMALS) was calculated in ASTRA 6.1 software (Wyatt) using a protein concentration determined from the refractive index signal with a dn/dc value of 0.185.
    ASTRA
    suggested: (ASTRA, RRID:SCR_016255)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • No funding statement was detected.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2020.09.08.286732v1 on bioRxiv