Deamidation drives molecular aging of the SARS-CoV-2 spike protein receptor-binding motif
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
No abstract available
Article activity feed
-
-
SciScore for 10.1101/2021.05.20.445042: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
NIH rigor criteria are not applicable to paper type.Table 2: Resources
Experimental Models: Cell Lines Sentences Resources HEK293F cells transfected with this construct were grown in a TubeSpin® bioreactor in FreeStyle293 medium for 72h at 37°C with 8% CO2 and agitation at 180 rpm. HEK293Fsuggested: NoneSoftware and Algorithms Sentences Resources To compare sites of deamidation, the protein sequences were aligned using Clustal Omega ( Clustal Omegasuggested: (Clustal Omega, RRID:SCR_001591)Data analysis: Raw data were searched against the Uniprot human (75,069 entries, June 2020) and the SARS-CoV-2 (14 entries, June 2020) reference proteome using MaxQuant 1.6.14.0 (31). MaxQuantsuggested: (MaxQuant, RRID:SCR…SciScore for 10.1101/2021.05.20.445042: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
NIH rigor criteria are not applicable to paper type.Table 2: Resources
Experimental Models: Cell Lines Sentences Resources HEK293F cells transfected with this construct were grown in a TubeSpin® bioreactor in FreeStyle293 medium for 72h at 37°C with 8% CO2 and agitation at 180 rpm. HEK293Fsuggested: NoneSoftware and Algorithms Sentences Resources To compare sites of deamidation, the protein sequences were aligned using Clustal Omega ( Clustal Omegasuggested: (Clustal Omega, RRID:SCR_001591)Data analysis: Raw data were searched against the Uniprot human (75,069 entries, June 2020) and the SARS-CoV-2 (14 entries, June 2020) reference proteome using MaxQuant 1.6.14.0 (31). MaxQuantsuggested: (MaxQuant, RRID:SCR_014485)txt was imported into Skyline 20.2.0.286 (55) Skylinesuggested: (Skyline, RRID:SCR_014080)MS1 spectra were inspected manually using Thermo Xcalibur Qual browser 4.2.28.14 Thermo Xcalibur Qual browsersuggested: NoneAll figures were prepared using R and ggplot2. ggplot2suggested: (ggplot2, RRID:SCR_014601)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
-