Deamidation drives molecular aging of the SARS-CoV-2 spike protein receptor-binding motif

  1. Ramiro Lorenzo
  2. Lucas A. Defelipe
  3. Lucio Aliperti
  4. Stephan Niebling
  5. Tânia F. Custódio
  6. Christian Löw
  7. Jennifer J. Schwarz
  8. Kim Remans
  9. Patricio O. Craig
  10. Lisandro H. Otero
  11. Sebastián Klinke
  12. María García-Alai
  13. Ignacio E. Sánchez
  14. Leonardo G. Alonso

Reviewed by

Read the full article

Listed in

Log in to save this article

Abstract

No abstract available

Article activity feed

  1. Version published to 10.1016/j.jbc.2021.101175
  2. ScreenIT

    SciScore for 10.1101/2021.05.20.445042: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    NIH rigor criteria are not applicable to paper type.

    Table 2: Resources

    Experimental Models: Cell Lines
    SentencesResources
    HEK293F cells transfected with this construct were grown in a TubeSpin® bioreactor in FreeStyle293 medium for 72h at 37°C with 8% CO2 and agitation at 180 rpm.
    HEK293F
    suggested: None
    Software and Algorithms
    SentencesResources
    To compare sites of deamidation, the protein sequences were aligned using Clustal Omega (
    Clustal Omega
    suggested: (Clustal Omega, RRID:SCR_001591)
    Data analysis: Raw data were searched against the Uniprot human (75,069 entries, June 2020) and the SARS-CoV-2 (14 entries, June 2020) reference proteome using MaxQuant 1.6.14.0 (31).
    MaxQuant
    suggested: (MaxQuant, RRID:SCR_014485)
    txt was imported into Skyline 20.2.0.286 (55)
    Skyline
    suggested: (Skyline, RRID:SCR_014080)
    MS1 spectra were inspected manually using Thermo Xcalibur Qual browser 4.2.28.14
    Thermo Xcalibur Qual browser
    suggested: None
    All figures were prepared using R and ggplot2.
    ggplot2
    suggested: (ggplot2, RRID:SCR_014601)

    Results from OddPub: Thank you for sharing your data.

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.05.20.445042v1 on bioRxiv