Arginine methylation of SARS-Cov-2 nucleocapsid protein regulates RNA binding, its ability to suppress stress granule formation, and viral replication
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SciScore for 10.1101/2021.03.24.436822: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources 1:2000); rabbit anti-SARS-CoV-2 N antibody (9103, Prosci, 1:2000); rabbit anti-SARS-CoV-2 S antibody (PA5-81795, Invitrogen, 1:1000); anti-SARS-CoV-2 Nsuggested: Noneanti-SARS-CoV-2 Ssuggested: Nonerabbit anti-TDRD3 antibody (Bethyl Laboratory, anti-TDRD3suggested: Nonemouse anti-SMN antibody (610646, BD Biosciences, 1:2000); rabbit anti-G3BP1 antibody (1F1, Rhône-Poulenc Rorer, 1:1000 anti-SMNsuggested: (BD Biosciences Cat# 610646, RRID:AB_397973)anti-G3BP1s…SciScore for 10.1101/2021.03.24.436822: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources 1:2000); rabbit anti-SARS-CoV-2 N antibody (9103, Prosci, 1:2000); rabbit anti-SARS-CoV-2 S antibody (PA5-81795, Invitrogen, 1:1000); anti-SARS-CoV-2 Nsuggested: Noneanti-SARS-CoV-2 Ssuggested: Nonerabbit anti-TDRD3 antibody (Bethyl Laboratory, anti-TDRD3suggested: Nonemouse anti-SMN antibody (610646, BD Biosciences, 1:2000); rabbit anti-G3BP1 antibody (1F1, Rhône-Poulenc Rorer, 1:1000 anti-SMNsuggested: (BD Biosciences Cat# 610646, RRID:AB_397973)anti-G3BP1suggested: NoneImmunofluorescence were performed with the following antibodies: mouse anti-Flag monoclonal antibody (F1804, Sigma Aldrich, 1:500); anti-Flagsuggested: (Sigma-Aldrich Cat# F1804, RRID:AB_262044)Alexa Fluor-conjugated goat anti-rabbit, goat anti-mouse secondary antibodies were from Invitrogen. anti-rabbitsuggested: Noneanti-mousesuggested: NoneExperimental Models: Cell Lines Sentences Resources Cell culture and transfection: HEK293 and VeroE6 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and grown at 37°C with 5% CO2. VeroE6suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)HEK293 and Huh-7 cells were transfected with plasmid DNAs by standard calcium phosphate precipitation and Lipofectamine 3000 (Invitrogen), respectively. HEK293suggested: NoneHuh-7suggested: NoneSoftware and Algorithms Sentences Resources rabbit anti-TDRD3 antibody (Bethyl Laboratory, Bethyl Laboratorysuggested: (Bethyl, RRID:SCR_013554)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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