Evaluating angiotensin-converting enzyme 2-mediated SARS-CoV-2 entry across species

Article activity feed

1. Version published to 10.1016/j.jbc.2021.100435

   Jan 1, 2021
2. 

   ScreenIT

   Jun 17, 2020

   SciScore for 10.1101/2020.06.15.153916: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   The nucleoprotein (N) of SARS-CoV was detected for infection and replication of the virus using an N-specific polyclonal antibody (Sinobiological, China), and a donkey anti-rabbit IgG (H+L) labeled with Cy3 (Jacksion, USA) was used as the secondary antibody.	anti-rabbit IgG suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Cells and SARS-CoV-2: HEK293T cells cells were maintained in DMEM (Gibco, USA) with 10% fetal bovine serum (HyClone, USA).	H…

   SciScore for 10.1101/2020.06.15.153916: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   The nucleoprotein (N) of SARS-CoV was detected for infection and replication of the virus using an N-specific polyclonal antibody (Sinobiological, China), and a donkey anti-rabbit IgG (H+L) labeled with Cy3 (Jacksion, USA) was used as the secondary antibody.	anti-rabbit IgG suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Cells and SARS-CoV-2: HEK293T cells cells were maintained in DMEM (Gibco, USA) with 10% fetal bovine serum (HyClone, USA).	HEK293T suggested: None
   Software and Algorithms
   Sentences	Resources
   Sequence analysis: ACE2 sequences of 12 different species were acquired from NCBI and their alignment were assessed using the ClustalW method in Lasergene software (Version 7.1) (DNASTAR Inc., USA).	ClustalW suggested: (ClustalW, RRID:SCR_017277)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on pages 20 and 21. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
3. 

   Version published to 10.1101/2020.06.15.153916v1 on bioRxiv

   Jun 16, 2020