Evaluating angiotensin-converting enzyme 2-mediated SARS-CoV-2 entry across species
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SciScore for 10.1101/2020.06.15.153916: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources The nucleoprotein (N) of SARS-CoV was detected for infection and replication of the virus using an N-specific polyclonal antibody (Sinobiological, China), and a donkey anti-rabbit IgG (H+L) labeled with Cy3 (Jacksion, USA) was used as the secondary antibody. anti-rabbit IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources Cells and SARS-CoV-2: HEK293T cells cells were maintained in DMEM (Gibco, USA) with 10% fetal bovine serum (HyClone, USA). H…SciScore for 10.1101/2020.06.15.153916: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources The nucleoprotein (N) of SARS-CoV was detected for infection and replication of the virus using an N-specific polyclonal antibody (Sinobiological, China), and a donkey anti-rabbit IgG (H+L) labeled with Cy3 (Jacksion, USA) was used as the secondary antibody. anti-rabbit IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources Cells and SARS-CoV-2: HEK293T cells cells were maintained in DMEM (Gibco, USA) with 10% fetal bovine serum (HyClone, USA). HEK293Tsuggested: NoneSoftware and Algorithms Sentences Resources Sequence analysis: ACE2 sequences of 12 different species were acquired from NCBI and their alignment were assessed using the ClustalW method in Lasergene software (Version 7.1) (DNASTAR Inc., USA). ClustalWsuggested: (ClustalW, RRID:SCR_017277)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on pages 20 and 21. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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