The Integrin Binding Peptide, ATN-161, as a Novel Therapy for SARS-CoV-2 Infection

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1. Version published to 10.1016/j.jacbts.2020.10.003

   Jan 1, 2021
2. 

   Version published to 10.1101/2020.06.15.153387v2 on bioRxiv

   Jul 24, 2020
3. 

   ScreenIT

   Jun 17, 2020

   SciScore for 10.1101/2020.06.15.153387: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Incubation with an HRP labeled goat anti-human Fc secondary antibody at 1:5000 for 30 minutes at 37□ was followed by detection by TMB substrate.	anti-human Fc secondary suggested: None
   In order to assess disruption of binding of α5β1 to SARS-CoV-2 Spike protein, 96-well plates were coated as before, but incubation with ATN-161 was performed in conjunction with 1μg/mL spike (produced under HHSN272201400008C and obtained through BEI Resources, NIAID, NIH: …

   SciScore for 10.1101/2020.06.15.153387: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Incubation with an HRP labeled goat anti-human Fc secondary antibody at 1:5000 for 30 minutes at 37□ was followed by detection by TMB substrate.	anti-human Fc secondary suggested: None
   In order to assess disruption of binding of α5β1 to SARS-CoV-2 Spike protein, 96-well plates were coated as before, but incubation with ATN-161 was performed in conjunction with 1μg/mL spike (produced under HHSN272201400008C and obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, Wuhan-Hu-1, Recombinant from HEK293 Cells, NR-52306) in the presence of 1mM MnCl2, followed by detection with an anti-spike antibody.	NIH: Spike Glycoprotein Receptor Binding Domain (RBD suggested: None anti-spike suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Cells and Virus: VeroE6 cells (ATCC# CRL-1586) were cultured in complete DMEM containing 10% fetal bovine serum (FBS).	VeroE6 suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)
   In order to assess disruption of binding of α5β1 to SARS-CoV-2 Spike protein, 96-well plates were coated as before, but incubation with ATN-161 was performed in conjunction with 1μg/mL spike (produced under HHSN272201400008C and obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, Wuhan-Hu-1, Recombinant from HEK293 Cells, NR-52306) in the presence of 1mM MnCl2, followed by detection with an anti-spike antibody.	HEK293 suggested: None
   Software and Algorithms
   Sentences	Resources
   The structures were rendered using PyMol 2.3.0 (36).	PyMol suggested: (PyMOL, RRID:SCR_000305)
   For IC50 estimation, the data points directly bounding the IC50 value were used and calculation was made in GraphPad Prism.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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4. 

   Version published to 10.1101/2020.06.15.153387v1 on bioRxiv

   Jun 16, 2020