Humoral immune responses against SARS-CoV-2 Spike variants after mRNA vaccination in solid organ transplant recipients
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SciScore for 10.1101/2022.05.13.22275056: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Cell surface staining and flow cytometry analysis: 293T cells were co-transfected with a GFP expressor (pIRES2-GFP, Clontech) in combination with plasmid encoding the full-length S of SARS-CoV-2 variants (D614G, Delta or Omicron) or the HCoV-HKU1 S. 293Tsuggested: NoneRecombinant DNA Sentences Resources Cell surface staining and flow cytometry analysis: 293T cells were co-transfected with a GFP expressor (pIRES2-GFP, Clontech) in combination with plasmid encoding … SciScore for 10.1101/2022.05.13.22275056: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Cell surface staining and flow cytometry analysis: 293T cells were co-transfected with a GFP expressor (pIRES2-GFP, Clontech) in combination with plasmid encoding the full-length S of SARS-CoV-2 variants (D614G, Delta or Omicron) or the HCoV-HKU1 S. 293Tsuggested: NoneRecombinant DNA Sentences Resources Cell surface staining and flow cytometry analysis: 293T cells were co-transfected with a GFP expressor (pIRES2-GFP, Clontech) in combination with plasmid encoding the full-length S of SARS-CoV-2 variants (D614G, Delta or Omicron) or the HCoV-HKU1 S. pIRES2-GFPsuggested: NoneVirus neutralization assay: To produce the pseudoviruses, 293T cells were transfected with the lentiviral vector pNL4.3 R-E-Luc (NIH AIDS Reagent Program) and a plasmid encoding for the indicated S glycoprotein (D614G, Delta or Omicron) at a ratio of 10:1. pNL4.3suggested: NoneSoftware and Algorithms Sentences Resources Samples were acquired on a LSRII cytometer (BD Biosciences), and data analysis was performed using FlowJo v10.7.1 (Tree Star). BD Biosciencessuggested: (BD Biosciences, RRID:SCR_013311)All samples were acquired on an LSRII cytometer (BD Biosciences) and data analysis was performed using FlowJo v10.7.1 (Tree Star). FlowJosuggested: (FlowJo, RRID:SCR_008520)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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