Functional evaluation of the P681H mutation on the proteolytic activation of the SARS-CoV-2 variant B.1.1.7 (Alpha) spike
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SciScore for 10.1101/2021.04.06.438731: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources SARS-CoV-2 S was detected using a rabbit polyclonal antibody against the S2 domain (Cat: 40590-T62, Sinobiological) and an AlexaFluor 488 goat anti-rabbit antibody. anti-rabbitsuggested: NoneSecondary antibody labeling was performed using AlexaFluorTM 488 goat anti-rabitt IgG antibody (Cat: A32731, Invitrogen Co.) at a 1/500 dilution for 45 minutes. anti-rabitt IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources Pseudoparticle Generation: … SciScore for 10.1101/2021.04.06.438731: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources SARS-CoV-2 S was detected using a rabbit polyclonal antibody against the S2 domain (Cat: 40590-T62, Sinobiological) and an AlexaFluor 488 goat anti-rabbit antibody. anti-rabbitsuggested: NoneSecondary antibody labeling was performed using AlexaFluorTM 488 goat anti-rabitt IgG antibody (Cat: A32731, Invitrogen Co.) at a 1/500 dilution for 45 minutes. anti-rabitt IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources Pseudoparticle Generation: HEK293T cells were seeded at 3×10e5 cells/ml in a 6 well plate the day before transfection. HEK293Tsuggested: NCBI_Iran Cat# C498, RRID:CVCL_0063)Pseudoparticle Infection Assay: Vero E6 and Vero TMPRSS2 cells were seeded at 3e5 cells/ml in a 24 well plate the day before infection. Vero E6suggested: NoneVero TMPRSS2suggested: NoneCell-Cell Fusion Assay: VeroE6 and Vero-TMPRSS2 cells were transfected with a plasmid harboring the spike gene of the SARS-CoV-2 isolate Wuhan-Hu 1, SARS-CoV-2 B.1.1.7 variant, the SARS-CoV-2 isolate Wuhan-Hu 1 with a P681H mutation, or a delta-spike pCDNA3.1+ plasmid, and evaluated through an immunofluorescence assay (IFA) Vero-TMPRSS2suggested: JCRB Cat# JCRB1818, RRID:CVCL_YQ48)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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