A realistic transfer method reveals low risk of SARS-CoV-2 transmission via contaminated euro coins and banknotes
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SciScore for 10.1101/2021.04.02.438182: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Preparation of test virus suspension: For preparation of SARS-CoV-2 test virus suspension, Vero E6 cells were seeded in a 75 cm2 flasks at 2×106 cells in Dulbecco’s Modified Eagle’s Medium (DMEM, supplemented with 10 % (v/v) fetal calf serum (FCS), 1 % non-essential amino acids, 100 IU/mL penicillin, 100 µg/mL streptomycin and 2 mM L-Glutamine). Vero E6suggested: NoneFor preparation of BCoV virus suspension, U373 cells were cultivated … SciScore for 10.1101/2021.04.02.438182: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Preparation of test virus suspension: For preparation of SARS-CoV-2 test virus suspension, Vero E6 cells were seeded in a 75 cm2 flasks at 2×106 cells in Dulbecco’s Modified Eagle’s Medium (DMEM, supplemented with 10 % (v/v) fetal calf serum (FCS), 1 % non-essential amino acids, 100 IU/mL penicillin, 100 µg/mL streptomycin and 2 mM L-Glutamine). Vero E6suggested: NoneFor preparation of BCoV virus suspension, U373 cells were cultivated in a 75 cm2 flask with in Minimum Essential Medium Eagle (EMEM) supplemented with L-glutamine, non-essential amino acids and sodium pyruvate and 10 % FCS. U373suggested: NoneSoftware and Algorithms Sentences Resources Fitting of virus titer decay: To model the decay in virus titer, we implemented a Weibull distribution fit in GraphPad Prism version 9.0.2 for Windows (GraphPad Software, San Diego, California USA, www.graphpad.com) Calculation of the reduction factor: The loss in virus titer by desiccation was calculated by subtracting the titer on the different carriers after desiccation from the titer of the initial virus control. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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