Graphene nanoplatelet and graphene oxide functionalization of face mask materials inhibits infectivity of trapped SARS-CoV-2
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SciScore for 10.1101/2020.09.16.20194316: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization After incubation five washes were carried out and images of random fields for each condition were acquired and the stain signal quantified as described below. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Further infection evaluation was done by labeling of SARS-CoV-2 particles with an anti-spike antibody and performing lactate dehydrogenase (LDH) assay, all described below. anti-spikesuggested: NoneLDHsuggested: NoneSARS-CoV-2 viral particles were labelled with monoclonal rabbit anti-Spike S1 subunit antibodies … SciScore for 10.1101/2020.09.16.20194316: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization After incubation five washes were carried out and images of random fields for each condition were acquired and the stain signal quantified as described below. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Further infection evaluation was done by labeling of SARS-CoV-2 particles with an anti-spike antibody and performing lactate dehydrogenase (LDH) assay, all described below. anti-spikesuggested: NoneLDHsuggested: NoneSARS-CoV-2 viral particles were labelled with monoclonal rabbit anti-Spike S1 subunit antibodies (Novusbio, clone CR3022), the plate was incubated for 3 hours at room temperature, and after washes with PBST, incubated with secondary anti-rabbit IgG – FITC labelled antibodies (Ref: 65-6111/ lot: UG285467, Invitrogen). anti-Spike S1 subunit antibodies ( Novusbio ,suggested: Noneanti-rabbit IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources Biocompatibility of functionalized materials: To assess if the cotton or PU materials functionalized with GNP or GO were toxic to cells in the absence of virus, A549 lung cancer cells (ATCC) were incubated with material-exposed medium. A549suggested: NCI-DTP Cat# A549, RRID:CVCL_0023)Software and Algorithms Sentences Resources Prior to perform the “Measure” tool of ImageJ, images were processed with the "Smooth" and "Convert to Mask". ImageJsuggested: (ImageJ, RRID:SCR_003070)Microsoft Excel (2010) and Prism6 software (GraphPad) were used to compile and analyze data. Microsoft Excelsuggested: (Microsoft Excel, RRID:SCR_016137)GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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