Characterization of SARS-CoV-2 nucleocapsid protein reveals multiple functional consequences of the C-terminal domain
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SciScore for 10.1101/2020.11.30.404905: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The collection of patient plasma was approved by the Human Research Protection Office at Washington University in St. Louis (IRB reference number 202007097) and the Institutional Review Board of The Hong Kong University and the Hong Kong Island West Cluster of Hospitals (IRB reference number UW20-169). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Following FBS blocking and thorough washing, diluted plasma samples (1:100) were bound for 2 hours, further washed and then detected by an anti – human IgG … SciScore for 10.1101/2020.11.30.404905: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The collection of patient plasma was approved by the Human Research Protection Office at Washington University in St. Louis (IRB reference number 202007097) and the Institutional Review Board of The Hong Kong University and the Hong Kong Island West Cluster of Hospitals (IRB reference number UW20-169). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Following FBS blocking and thorough washing, diluted plasma samples (1:100) were bound for 2 hours, further washed and then detected by an anti – human IgG secondary antibody labelled with HRP (Invitrogen), and absorbance detected at 450nm on a spectrophotometer (Wallac) anti – human IgGsuggested: Nonehumansuggested: NoneExperimental Models: Cell Lines Sentences Resources IFN-β promoter reporter gene assay: HEK-293T cells (5 × 104) were co-transfected using Lipofectamine 2000 with 25 ng of an IFN-β promoter-firefly luciferase reporter plasmid, 25 ng of pRL-TK Renilla luciferase reporter plasmid, and 125, 12.5, and 1.25 ng of the indicated viral protein expression plasmid. HEK-293Tsuggested: NoneSoftware and Algorithms Sentences Resources Fluorescence Polarization Assay (FPA): FPA experiments were performed on a Cytation5 plate reader (BioTek) operating on Gen5 software. Gen5suggested: (Gen5, RRID:SCR_017317)The fluorescence polarization values were then plotted against N concentrations to fit the dissociation constant, KD, using ORIGIN software. ORIGINsuggested: (Origin, RRID:SCR_014212)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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