Transcriptomic profiling of SARS-CoV-2 infected human cell lines identifies HSP90 as target for COVID-19 therapy
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SciScore for 10.1101/2020.05.05.079194: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Primary detection of hACE-2 was done using a goat anti-hACE-2 antibody (1:1,250; #AF933, R&D Systems), a horseradish peroxidase (HRP)-labeled donkey anti-goat antibody (1:5,000, Dianova) and Super Signal West Femto Chemiluminescence Substrate (Thermo Fisher Scientific). anti-hACE-2suggested: NoneHRP)-labeled donkey anti-goat antibody (1:5,000, Dianova)suggested: NoneAs loading control, samples were analyzed for β-actin expression using a mouse anti-β-actin … SciScore for 10.1101/2020.05.05.079194: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Primary detection of hACE-2 was done using a goat anti-hACE-2 antibody (1:1,250; #AF933, R&D Systems), a horseradish peroxidase (HRP)-labeled donkey anti-goat antibody (1:5,000, Dianova) and Super Signal West Femto Chemiluminescence Substrate (Thermo Fisher Scientific). anti-hACE-2suggested: NoneHRP)-labeled donkey anti-goat antibody (1:5,000, Dianova)suggested: NoneAs loading control, samples were analyzed for β-actin expression using a mouse anti-β-actin antibody (1:5,000, Sigma Aldrich) and a HRP-labeled goat anti-mouse antibody (1:10,000, Sigma-Aldrich). anti-β-actinsuggested: Noneanti-mousesuggested: NoneExperimental Models: Cell Lines Sentences Resources (ATCC HTB-37) and H1299 (ATCC CRL-5803) were cultivated in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% heat-inactivated fetal calf serum, 1% non-essential amino acids, 1% L-glutamine and 1% sodium pyruvate (all Thermo Fisher Scientific) in a 5% CO2 atmosphere at 37 °C. H1299suggested: ATCC Cat# CRL-5803, RRID:CVCL_0060)Cells inoculated with cell culture supernatants from uninfected Vero cells mixed with OptiPro serum-free medium supplemented with 0.5% gelatine and PBS, in accordance to virus stock preparation, serves as mock infected controls. Verosuggested: CLS Cat# 605372/p622_VERO, RRID:CVCL_0059)For the assay Vero E6 cells were seeded to confluence and infected with serial dilution of virus-containing cell culture supernatant diluted in OptiPro serum-free medium. Vero E6suggested: RRID:CVCL_XD71)Infections for RNA sequencing experiments: Calu-3 cells and H1299 cells were seeded at a concentration of 6 x 10^5 cells/mL and 5 x 10^4 cells/mL, respectively. Calu-3suggested: NoneResults from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
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