The impacts of vaccination status and host factors during early infection on SARS-CoV-2 persistence: a retrospective single-center cohort study

  1. Xiangxiang Tian
  2. Yifan Zhang
  3. Wanhai Wang
  4. Fang Fang
  5. Wenhong Zhang
  6. Zhaoqin Zhu
  7. Yanmin Wan

Reviewed by

Read the full article See related articles

Listed in

Log in to save this article

Abstract

No abstract available

Article activity feed

  1. Version published to 10.1016/j.intimp.2022.109534
  2. ScreenIT

    SciScore for 10.1101/2022.04.14.22273860: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    EthicsIRB: Ethics statement: This study was approved by the Research Ethics Review Committee (Ethics Approval Number: YJ-2020-S080-02) of the Shanghai Public Health Clinical Center Affiliated to Fudan University.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.

    Table 2: Resources

    Software and Algorithms
    SentencesResources
    Bioperfectus technologies Co., LTD., Jiangsu, China) and ABI 7500 real-time fluorescence quantitative PCR (qPCR) instrument (Thermo Fisher Technology Co.
    Thermo Fisher Technology Co
    suggested: None
    All the statistical analyses were conducted using Graphpad Prism 9 (GraphPad Software, USA).
    Graphpad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)
    GraphPad
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:
    Several limitations of the present study should be noted. First, antigen-specific immune responses were not detected in this retrospective work. Nonetheless, we speculate that the vaccine induce immunities might not be able to constrain viral RNA shedding in mild COVID-19 patients, since both an inactivated vaccine and an mRNA vaccine failed to shorten the duration of viral RNA shedding in these patients. Second, although a previous study showed that viral RNA load (>107 copies/ml) is an independent risk factor for live virus shedding (35), the detection viral RNA might not always indicate the presence of live viruses. Further investigations are necessitated to characterize host factors that associate with the shedding of viable viruses. Third, most cases enrolled in this study were infected by either the D614G variant or the Delta variant, but we were not able to define the infected variant for each case. Therefore, the impact of viral factor on the duration of viral RNA shedding is out-of-scope for this study. Despite of these limitations, our study showed for the first time that COVID-19 vaccines contributed to the clearance viral RNA in moderate cases, while failed to shorten the duration of viral RNA shedding in mild patients. Moreover, we identified a set of laboratory variables in early infection that could predict the persistence of viral RNA. These findings may serve as a rationale to optimize the control measures for COVID-19 pandemic.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a protocol registration statement.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2022.04.14.22273860v1 on medRxiv