Low-Avidity CD4+ T Cell Responses to SARS-CoV-2 in Unexposed Individuals and Humans with Severe COVID-19
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Abstract
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SciScore for 10.1101/2020.09.15.20188896: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources In brief, 0.5-2×10e7 PBMCs were plated in RPMI-1640 medium (GIBCO), supplemented with 5% (v/v) human AB-serum (Sigma Aldrich, Schnelldorf, Germany) at a cell density of 1×10e7 PBMCs / 2 cm2 in cell culture plates and stimulated for 7 hr in presence of 1 μg/ml CD40 and 1 μg/ml CD28 pure antibody (both Miltenyi Biotec, Bergisch Gladbach, Germany). CD40suggested: NoneCD28suggested: NoneSoftware and Algorithms Sentences Resources FlowJo (Treestar, Ashland, OR, USA) software was used for analysis. Fl…SciScore for 10.1101/2020.09.15.20188896: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources In brief, 0.5-2×10e7 PBMCs were plated in RPMI-1640 medium (GIBCO), supplemented with 5% (v/v) human AB-serum (Sigma Aldrich, Schnelldorf, Germany) at a cell density of 1×10e7 PBMCs / 2 cm2 in cell culture plates and stimulated for 7 hr in presence of 1 μg/ml CD40 and 1 μg/ml CD28 pure antibody (both Miltenyi Biotec, Bergisch Gladbach, Germany). CD40suggested: NoneCD28suggested: NoneSoftware and Algorithms Sentences Resources FlowJo (Treestar, Ashland, OR, USA) software was used for analysis. FlowJosuggested: (FlowJo, RRID:SCR_008520)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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