Age significantly influences the sensitivity of SARS-CoV-2 rapid antibody assays

  1. Natalie Irwin
  2. Lyle Murray
  3. Benjamin Ozynski
  4. Guy A Richards
  5. Graham Paget
  6. Jacqueline Venturas
  7. Ismail Kalla
  8. Nina Diana
  9. Adam Mahomed
  10. Jarrod Zamparini

Reviewed by

Read the full article See related articles

Listed in

Log in to save this article

Abstract

No abstract available

Article activity feed

  1. Version published to 10.1016/j.ijid.2021.07.027
  2. ScreenIT

    SciScore for 10.1101/2021.01.28.21250675: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIRB: This study was approved by the University of the Witwatersrand Human Research Ethics Committee (Medical) (M200697).
    Consent: Written informed consent was obtained from all participants and patient data were anonymised prior to analysis.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.

    Table 2: Resources

    Software and Algorithms
    SentencesResources
    Clinical and biographical data were collected using an electronic database (REDCap 10.6.2, Vanderbilt University) by means of a self-administered participant questionnaire.
    REDCap
    suggested: (REDCap, RRID:SCR_003445)
    Statistical analysis: Data were analysed using Prism 8.4.3 (GraphPad Software Inc, La Jolla, California) using standard non-parametric statistical tests as appropriate.
    Prism
    suggested: (PRISM, RRID:SCR_005375)
    GraphPad
    suggested: (GraphPad Prism, RRID:SCR_002798)
    The multivariate logistic regression analysis was performed using IBM SPSS Statistics
    SPSS
    suggested: (SPSS, RRID:SCR_002865)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:
    There are limitations to this study. Although used as the reference gold standard, PCR-based testing gives no indication of patient seropositivity. The negative results reported may represent low antibody titres or low participant seroconversion, rather than a failure of the test to detect antibodies. Furthermore, two assays (Innovita and Cellex) that were included have not previously been validated on finger-prick blood samples. Although we consider the effect is likely to be small, as all other tests could be used across blood sample platforms, this may have contributed to their poor performance. Owing to inadequate access to negative control samples, we were not able to perform a corresponding specificity analysis.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.01.28.21250675v1 on medRxiv