Less severe course of COVID-19 is associated with elevated levels of antibodies against seasonal human coronaviruses OC43 and HKU1 (HCoV OC43, HCoV HKU1)
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Abstract
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Francisco Ibarrondo
Review 1: "Less severe course of COVID-19 is associated with elevated levels of antibodies against seasonal human coronaviruses OC43 and HKU1 (HCoV OC43, HCoV HKU1"
Reviewer: Francisco Ibarrondo (UCLA) | 📙📙 ◻️◻️◻️
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Francisco Ibarrondo
Review of "Less severe course of COVID-19 is associated with elevated levels of antibodies against seasonal human coronaviruses OC43 and HKU1 (HCoV OC43, HCoV HKU1"
Reviewer: Francisco Ibarrondo (UCLA) | 📙📙 ◻️◻️◻️
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SciScore for 10.1101/2020.10.12.20211599: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Patient cohort: Serum samples from 60 Patients with COVID-19 infections confirmed by RT-qPCR were analyzed in the context of the Coronaplasma Project (local ethics committee approval: AZ 2020-220-f-S) and COVID-19 biomarker study (local ethics committee approval: AZ 2020-210-f-S) at the University Hospital of Münster, Germany.
Consent: After informed consent, serum was collected at the first patient contact.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable 52 males and 8 females were included. Table 2: Resources
Antibodies Sentences Resources Regarding seasonal coronaviruses, this assay measures IgG antibodies … SciScore for 10.1101/2020.10.12.20211599: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Patient cohort: Serum samples from 60 Patients with COVID-19 infections confirmed by RT-qPCR were analyzed in the context of the Coronaplasma Project (local ethics committee approval: AZ 2020-220-f-S) and COVID-19 biomarker study (local ethics committee approval: AZ 2020-210-f-S) at the University Hospital of Münster, Germany.
Consent: After informed consent, serum was collected at the first patient contact.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable 52 males and 8 females were included. Table 2: Resources
Antibodies Sentences Resources Regarding seasonal coronaviruses, this assay measures IgG antibodies directed against the nucleocapsid protein (NP) of HCoV 229E, NL63, OC43 and HKU1. NL63suggested: NoneHKU1suggested: NoneSoftware and Algorithms Sentences Resources In addition, relative antibody levels were quantitatively determined with ImageJ (version 153, 64bit-Version for windows) [6] using the signal intensity of the cutoff band of individual immunstrips as internal reference. ImageJsuggested: (ImageJ, RRID:SCR_003070)Demographical data, type of treatment and length of stay were extracted by the Medical Data Integration Center (MeDIC) from the hospital information system of the University Hospital Münster (ORBIS from Dedalus Healthcare Group). Dedalus Healthcaresuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:This study has important limitations: It is a retrospective single-site study with a limited number of cases and association is not causation. However, it is remarkable that the effect of HCoV OC43 and HKU1-specific antibody levels reached statistical significance regarding the need for intensive care therapy with only 60 patients. Therefore, these findings should be validated in other sites with larger patient collectives. In a prospective setting (e.g. for risk groups with contacts to many persons like employees of hospitals or supermarkets) it should be tested if the absence of HCoV OC43 and HKU1-specific antibody levels can identify persons at risk for a severe course of COVID-19. Identification of vulnerable individuals is a key priority in the current stage of the pandemic to guide protective measures and to design vaccination strategies.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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SciScore for 10.1101/2020.10.12.20211599: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Regarding seasonal coronaviruses, this assay measures IgG antibodies directed against the nucleocapsid protein (NP) of HCoV 229E, NL63, OC43 and HKU1. NL63suggested: NoneHKU1suggested: NoneA two-sided p-value of 0.05 was considered significant. 3: a) … SciScore for 10.1101/2020.10.12.20211599: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Regarding seasonal coronaviruses, this assay measures IgG antibodies directed against the nucleocapsid protein (NP) of HCoV 229E, NL63, OC43 and HKU1. NL63suggested: NoneHKU1suggested: NoneA two-sided p-value of 0.05 was considered significant. 3: a) Correlation of length of stay and OC43 antibody levels. OC43suggested: (Abcam Cat# ab28169, RRID:AB_777848)Software and Algorithms Sentences Resources In addition, relative antibody levels were quantitatively determined with ImageJ (version 153, 64bit-Version for windows) [6] using the signal intensity of the cutoff band of individual immunstrips as internal reference. ImageJsuggested: (ImageJ, RRID:SCR_003070)Demographical data, type of treatment and length of stay were extracted by the Medical Data Integration Center (MeDIC) from the hospital information system of the University Hospital Münster (ORBIS from Dedalus Healthcare Group). Dedalus Healthcaresuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:
This study has important limitations: It is a retrospective single-site study with a limited number of cases and association is not causation. However, it is remarkable that the effect of HCoV OC43 and HKU1specific antibody levels reached statistical significance regarding the need for intensive care therapy with only 60 patients. Therefore, these findings should be validated in other sites with larger patient collectives. In a prospective setting (e.g. for risk groups with contacts to many persons like employees of hospitals or supermarkets) it should be tested if the absence of HCoV OC43 and HKU1-specific antibody levels can identify persons at risk for a severe course of COVID-19. Identification of vulnerable individuals is a key priority in the current stage of the pandemic to guide protective measures and to design vaccination strategies.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
About SciScore
SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.
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