The effect of ultraviolet C radiation against different N95 respirators inoculated with SARS-CoV-2
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SciScore for 10.1101/2020.05.31.20118588: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Viral stocks of SARS-COV-2 were obtained from the Biodefense and Emerging Infections Research Resources Repository and were propagated in Vero-E6 cells grown in Dulbecco’s Modified Eagle Medium (DMEM) without phenol red, with 2% Fetal Bovine Serum (FBS), L-glutamine, penicillin/streptomycin, non-essential amino acids, and hydroxyethyl piperazineethanesulfonic acid (HEPES). Vero-E6suggested: None(6) However, an absence of CPE in the … SciScore for 10.1101/2020.05.31.20118588: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Viral stocks of SARS-COV-2 were obtained from the Biodefense and Emerging Infections Research Resources Repository and were propagated in Vero-E6 cells grown in Dulbecco’s Modified Eagle Medium (DMEM) without phenol red, with 2% Fetal Bovine Serum (FBS), L-glutamine, penicillin/streptomycin, non-essential amino acids, and hydroxyethyl piperazineethanesulfonic acid (HEPES). Vero-E6suggested: None(6) However, an absence of CPE in the Vero E6 cells at 4 days post inoculation indicates a loss of infectivity and is evidence of inactivation of the SARS-CoV-2 samples (Figure 1B). Vero E6suggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Limitations of the study include that no soiling agents were used. However, at Henry Ford Health System, as in other healthcare facilities, personnel are instructed not to reuse respirators that are visibly soiled. Further, it is still unclear what the infectious dose is for SARS-CoV-2; therefore, it is unknown if a significant reduction in viral load eliminates contagious risk. In conclusion, UVC at a dose of 1.5 J/cm2 applied to both sides is effective at decontaminating SARS-CoV-2 on some N95 respirators. This dose may only be an appropriate decontamination method to facilitate reuse of PPE for healthcare personnel when applied to certain models/materials. In addition, some straps may require additional disinfection to maximize the safety to the frontline workers. Implementation of widespread UVC decontamination methods requires a careful consideration of model, material type, design, and fit-testing following irradiation. It should also be emphasized that similar cautions should be practiced for all other methods of respirator decontamination.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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