Lack of antiviral activity of darunavir against SARS-CoV-2

Article activity feed

1. Version published to 10.1016/j.ijid.2020.05.085

   Aug 1, 2020
2. 

   ScreenIT

   Jun 5, 2020

   SciScore for 10.1101/2020.04.03.20052548: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   After one passage in Caco-2 cells, viral stocks were stored at –80°C prior to use.	Caco-2 suggested: None
   Assessment of Cell Viability: To assess the effects of the compounds on Caco–2 cell viability, cell viability was measured in confluent cell layers treated with a range of compound concentrations in absence of virus using the Rotitest Vital (Roth) according to manufacturer’s instructions, as previously described (12).	Caco–2 suggested…

   SciScore for 10.1101/2020.04.03.20052548: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   After one passage in Caco-2 cells, viral stocks were stored at –80°C prior to use.	Caco-2 suggested: None
   Assessment of Cell Viability: To assess the effects of the compounds on Caco–2 cell viability, cell viability was measured in confluent cell layers treated with a range of compound concentrations in absence of virus using the Rotitest Vital (Roth) according to manufacturer’s instructions, as previously described (12).	Caco–2 suggested: None
   Software and Algorithms
   Sentences	Resources
   Data were analyzed by a four-parameter curve-fitting from a dose-response curve using GraphPad Prism (version 7.00) to calculate the EC50 (concentration of the compound that inhibited 50% of the infection) based on visual CPE scoring or based on the MTT method.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
3. 

   Version published to 10.1101/2020.04.03.20052548v1 on medRxiv

   Apr 8, 2020