The method utilized to purify the SARS-CoV-2 N protein can affect its molecular properties
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SciScore for 10.1101/2021.05.03.442392: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Recombinant DNA Sentences Resources The purified PCR product was cloned into the pET-SUMO vector digested with NcoI and NotI restriction enzymes. pET-SUMOsuggested: RRID:Addgene_73261)The final construct (pET-SUMO/N) was confirmed by DNA sequencing. pET-SUMO/Nsuggested: NoneSoftware and Algorithms Sentences Resources The parameters of the N protein, either fused or not fused to 6×His-SUMO, were calculated using the ProtParam server (Gasteiger et al, 2005). ProtParamsuggested: (ProtParam Tool, RRID:SCR_018087)Buffer density (ρ = 1.0121 g/cm3) and viscosity … SciScore for 10.1101/2021.05.03.442392: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Recombinant DNA Sentences Resources The purified PCR product was cloned into the pET-SUMO vector digested with NcoI and NotI restriction enzymes. pET-SUMOsuggested: RRID:Addgene_73261)The final construct (pET-SUMO/N) was confirmed by DNA sequencing. pET-SUMO/Nsuggested: NoneSoftware and Algorithms Sentences Resources The parameters of the N protein, either fused or not fused to 6×His-SUMO, were calculated using the ProtParam server (Gasteiger et al, 2005). ProtParamsuggested: (ProtParam Tool, RRID:SCR_018087)Buffer density (ρ = 1.0121 g/cm3) and viscosity (η = 0.010447 mPa×s) were calculated from the composition of the buffer using SEDNTERP software (Harding, 1992). SEDNTERPsuggested: (Sednterp, RRID:SCR_016253)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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