Inhibition of the replication of SARS-CoV-2 in human cells by the FDA-approved drug chlorpromazine
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SciScore for 10.1101/2020.05.05.079608: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Cell culture and virus isolates: Vero E6 cells (African green monkey kidney epithelial cells, ATCC, CRL-1586) were maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) and 5 units/mL penicillin and 5 μg/mL streptomycin at 37°C with 5% CO2. Vero E6suggested: NoneA549-ACE2 cells (adenocarcinomic human alveolar basal epithelial cells, transduced to express the human Angiotensin-converting enzyme … SciScore for 10.1101/2020.05.05.079608: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Cell culture and virus isolates: Vero E6 cells (African green monkey kidney epithelial cells, ATCC, CRL-1586) were maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) and 5 units/mL penicillin and 5 μg/mL streptomycin at 37°C with 5% CO2. Vero E6suggested: NoneA549-ACE2 cells (adenocarcinomic human alveolar basal epithelial cells, transduced to express the human Angiotensin-converting enzyme 2 (ACE2), kind gift of Pr. O. Schwartz, Institut Pasteur, Paris France) were maintained in DMEM containing 10% FBS, 5 units/mL penicillin and 5 μg/mL streptomycin and 40 µg/mL blasticidin at 37°C with 5% CO2. A549-ACE2suggested: NoneSoftware and Algorithms Sentences Resources IC50 values were fitted using four-parameter dose response curves in GraphPad prism v8.4.2. GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: We found the following clinical trial numbers in your paper:
Identifier Status Title NCT04366739 Not yet recruiting Repurposing of Chlorpromazine in Covid-19 Treatment Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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