Elevated Neutrophil Gelatinase-Associated Lipocalin Is Associated With the Severity of Kidney Injury and Poor Prognosis of Patients With COVID-19

  1. Katherine Xu
  2. Ning Shang
  3. Abraham Levitman
  4. Alexa Corker
  5. Satoru Kudose
  6. Andrew Yaeh
  7. Uddhav Neupane
  8. Jacob Stevens
  9. Rosemary Sampogna
  10. Angela M. Mills
  11. Vivette D’Agati
  12. Sumit Mohan
  13. Krzysztof Kiryluk
  14. Jonathan Barasch

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Abstract

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  1. Version published to 10.1016/j.ekir.2021.09.005
  2. ScreenIT

    SciScore for 10.1101/2021.06.10.21258638: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    EthicsIRB: Oversight of Human and Animal Studies: The Columbia University Biobank COVID-19 studies were reviewed and approved by the Columbia University Medical Center Institutional Review Board (IRB).
    IACUC: Mice were utilized according to the Institutional Animal Care and Use Committee (AC-AAAY7464) and adhere to NIH Guide for the Care and Use of Laboratory Animals.
    Sex as a biological variableRenal Ischemia Reperfusion Injury Model: Male and female wild-type C57Bl/6 mice, aged 8-10 weeks (Jackson Labs) were anesthetized with isoflurane and placed on a warming table to maintain a rectal temperature of 37°C.
    Randomizationnot detected.
    BlindingMeasurements of uNGAL, uKIM-1, and proteinuria were blinded from clinical data.
    Power Analysisnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Urinary cell pellet Western blots: Urinary cell pellets were analyzed for LRP2 and UMOD by immunoblot with SDS-PAGE (Bio-rad Laboratories), rabbit anti-LRP2 (1:1000, Abcam, ab76969), sheep anti-UMOD (1:2000, Meridian Life Science, K90071C), and polyclonal secondary antibodies conjugated to HRP (1:10,000, Jackson Immuno-Research).
    anti-LRP2
    suggested: (Abcam Cat# ab76969, RRID:AB_10673466)
    anti-UMOD
    suggested: None
    K90071C
    suggested: (Meridian Life Science Cat# K90071C, RRID:AB_153128)
    HRP
    suggested: None
    Experimental Models: Organisms/Strains
    SentencesResources
    Renal Ischemia Reperfusion Injury Model: Male and female wild-type C57Bl/6 mice, aged 8-10 weeks (Jackson Labs) were anesthetized with isoflurane and placed on a warming table to maintain a rectal temperature of 37°C.
    C57Bl/6
    suggested: None
    Recombinant DNA
    SentencesResources
    All statistical analyses were performed using R (CRAN version 4.0.4), including R add-on packages MASS (version 7.3-53.1), odds ratio (version 2.0.1), survival (version 3.2-7), cmprsk (version 2.2-10), and pROC (version 1.17. 0.1).
    pROC
    suggested: None
    Software and Algorithms
    SentencesResources
    All statistical analyses were performed using R (CRAN version 4.0.4), including R add-on packages MASS (version 7.3-53.1), odds ratio (version 2.0.1), survival (version 3.2-7), cmprsk (version 2.2-10), and pROC (version 1.17. 0.1).
    CRAN
    suggested: (CRAN, RRID:SCR_003005)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.06.10.21258638v1 on medRxiv