A cyclic peptide inhibitor of the SARS-CoV-2 main protease
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SciScore for 10.1101/2020.08.03.234872: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources HEK-293 cells were plated in a 96-well plate at 15,000 cells per well. HEK-293suggested: NoneSoftware and Algorithms Sentences Resources The IC50 value for UCI-1 was determined using the GraphPad Prism 8.4.3 software (GraphPad) by plotting the initial rates. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798): Docking of UCI-1 to SARS-CoV-2 Mpro: The model of the SARS-CoV-2 Mpro …
SciScore for 10.1101/2020.08.03.234872: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources HEK-293 cells were plated in a 96-well plate at 15,000 cells per well. HEK-293suggested: NoneSoftware and Algorithms Sentences Resources The IC50 value for UCI-1 was determined using the GraphPad Prism 8.4.3 software (GraphPad) by plotting the initial rates. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798): Docking of UCI-1 to SARS-CoV-2 Mpro: The model of the SARS-CoV-2 Mpro was generated as follows: Starting coordinates of SARS-CoV-2 Mpro were generated from the SARS-CoV-2 Mpro crystallographic structure (PDB 6YB7) using PyMOL and saved as a new PDB file.
PyMOLsuggested: (PyMOL, RRID:SCR_000305)The lowest energy cluster, as determined by AutoDock Vina was chosen to represent the SARS-CoV-2 Mpro UCI-1 interaction model in Figure 7C. AutoDocksuggested: (AutoDock, RRID:SCR_012746)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
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