Centenarians and extremely old people living with frailty can elicit durable SARS-CoV-2 spike specific IgG antibodies with virus neutralization functions following virus infection as determined by serological study

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1. Version published to 10.1016/j.eclinm.2021.100975

   Jul 1, 2021
2. 

   ScreenIT

   Mar 15, 2021

   SciScore for 10.1101/2021.03.05.21252707: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Secondary antibody was diluted in 1% BSA in PBS-T as follows: anti-human IgG-HRP 1:2000 (Invitrogen 31413)	anti-human IgG-HRP suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   For S protein production, Mexi293E cells were grown in 6 well cell culture plates.	Mexi293E suggested: None
   The virus was amplified in Vero-76 cells in viral growth media vDMEM (Dulbecco’s Modified Eagle Medium (Wisent Bioproducts (Cat # 319-005-CL)), 2% fetal calf serum …

   SciScore for 10.1101/2021.03.05.21252707: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Secondary antibody was diluted in 1% BSA in PBS-T as follows: anti-human IgG-HRP 1:2000 (Invitrogen 31413)	anti-human IgG-HRP suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   For S protein production, Mexi293E cells were grown in 6 well cell culture plates.	Mexi293E suggested: None
   The virus was amplified in Vero-76 cells in viral growth media vDMEM (Dulbecco’s Modified Eagle Medium (Wisent Bioproducts (Cat # 319-005-CL)), 2% fetal calf serum (Wisent Bioproducts (Cat # 090-150)), 5 mL 100x Penicillin (10,000 U/mL)/Streptomycin (10,000 ug/mL), and 2 mg/mL TPCK-trypsin) at VIDO, Saskatoon, Saskatchewan.	Vero-76 suggested: None
   Neutralization Assay: Vero cells (ATCC® CRL-1587) were seeded into 96-well tissue culture plates (Corning) at 20,000 cells per well and incubated overnight at 37°C in a 5% CO2 chamber.	Vero suggested: None
   The plasma-virus mixture was added to plated Vero 76 cells and incubated at 37°C in a 5% CO2 chamber for 1 h for virus absorption.	Vero 76 suggested: None
   Software and Algorithms
   Sentences	Resources
   Statistical Analysis: Results were analysed using GraphPad Prism8.	GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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3. 

   Version published to 10.1101/2021.03.05.21252707 on medRxiv

   Mar 8, 2021