Increased resistance of SARS-CoV-2 Omicron variant to neutralization by vaccine-elicited and therapeutic antibodies
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
Article activity feed
-
-
SciScore for 10.1101/2021.12.28.474369: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Consent: Human sera and monoclonal antibodies: Human sera were collected at the NYU Vaccine Center with written consent of participants under IRB-approved protocols 18-02035 and 18-02037. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Cells: 293T, ACE2.293T and Vero cells were grown in Dulbecco’s Modified Eagle’s Medium/10% fetal bovine serum at 37°C under 5% CO2. Verosuggested: NoneSARS-CoV-2 spike protein lentiviral pseudotypes: Spike protein pseudotyped lentiviruses were produced by cotransfection of 293T cells with pMDL … SciScore for 10.1101/2021.12.28.474369: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Consent: Human sera and monoclonal antibodies: Human sera were collected at the NYU Vaccine Center with written consent of participants under IRB-approved protocols 18-02035 and 18-02037. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Cells: 293T, ACE2.293T and Vero cells were grown in Dulbecco’s Modified Eagle’s Medium/10% fetal bovine serum at 37°C under 5% CO2. Verosuggested: NoneSARS-CoV-2 spike protein lentiviral pseudotypes: Spike protein pseudotyped lentiviruses were produced by cotransfection of 293T cells with pMDL Gag/Pol packaging vector, lentiviral vector plenti. 293Tsuggested: KCB Cat# KCB 200744YJ, RRID:CVCL_0063)Recombinant DNA Sentences Resources Plasmids: Plasmid expression vectors used in the production of lentiviral pseudotypes pMDL, pcVSV.G, pRSV. pRSVsuggested: RRID:Addgene_106453)Rev and the lentiviral dual reporter virus genome pLenti. pLentisuggested: RRID:Addgene_125133)The full-length coding sequence was generated by overlap extension PCR with the two fragments amplified with external primers containing a Kpn-I and Xho-I sites and then cloned into pcDNA6 pcDNA6suggested: RRID:Addgene_134280)Expression vectors encoding spike proteins with the individual mutations of the Omicron spike protein were generated by overlap extension PCR mutagenesis using the D614G spike protein plasmid pcCOV2.Δ19.D614G as template. pcCOV2.Δ19.D614Gsuggested: NoneSARS-CoV-2 spike protein lentiviral pseudotypes: Spike protein pseudotyped lentiviruses were produced by cotransfection of 293T cells with pMDL Gag/Pol packaging vector, lentiviral vector plenti. pMDL Gag/Polsuggested: NoneSoftware and Algorithms Sentences Resources Data were analyzed using GraphPad Prism 8 software and statistical significance was determined by the two-tailed unpaired t-test or nonparametric ANOVA test. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Analyses of the structures of the SARS-CoV-2 spike protein with antibody Fabs was performed with the PyMOL Molecular Graphics System, PyMOLsuggested: (PyMOL, RRID:SCR_000305)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Study Limitations: This study was done on a relatively small number of participants which limits the resolution of fine difference in antibody titers in the different groups. In addition, it depends entirely on pseudotyped viruses rather than antibody neutralization of live virus. While pseudotyped virus has been shown to provide similar data to that of the live virus assays, it is conceivable that there could be differences [30].
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
-